Difference between revisions of "Team:Warwick/Project"
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<br>1) A new device that enables cells which can ‘stick’ and be directed to an area | <br>1) A new device that enables cells which can ‘stick’ and be directed to an area | ||
<br>2) A target to which the cells can then attach to. | <br>2) A target to which the cells can then attach to. | ||
| − | <br>Constructing cells with the ability to recognise and attach to a target is achievable in the realm of synthetic biology; by modifying a cell to express and display zinc finger proteins on the outer surface membrane. | + | <br>Constructing cells with the ability to recognise and attach to a target is achievable in the realm of synthetic biology; by modifying a cell to express and display zinc finger proteins on the outer surface membrane. </p |
| − | <img src="https://static.igem.org/mediawiki/2015/3/3d/Warwick_diagram_of_redirecting_protein.jpeg"> | + | <p> |
| + | <img src="https://static.igem.org/mediawiki/2015/3/3d/Warwick_diagram_of_redirecting_protein.jpeg"> </p> | ||
| + | <p> | ||
The target to which the zinc finger binds is to a sequence of DNA. The DNA will be arranged in a shape, as inspired by concepts from DNA origami, to fold into a planar shape with long extending arms. The end of the DNA arms will contain thee target sequences, to which the cells can adhere to. The arms of DNA origami structure can be designed and programmed to contain a variety of target regions therefore a combination of cells with differing zinc fingers can be brought together in an area. | The target to which the zinc finger binds is to a sequence of DNA. The DNA will be arranged in a shape, as inspired by concepts from DNA origami, to fold into a planar shape with long extending arms. The end of the DNA arms will contain thee target sequences, to which the cells can adhere to. The arms of DNA origami structure can be designed and programmed to contain a variety of target regions therefore a combination of cells with differing zinc fingers can be brought together in an area. | ||
| + | </p> | ||
| + | <p> | ||
<img src="https://static.igem.org/mediawiki/2015/1/14/Warwick_Diagram_of_specific_zinc_finger_DNA_binding_2.jpeg"> | <img src="https://static.igem.org/mediawiki/2015/1/14/Warwick_Diagram_of_specific_zinc_finger_DNA_binding_2.jpeg"> | ||
| − | < | + | </p> |
| − | + | <p> | |
| − | + | The inspiration from this project has come from a paper on ‘barcoding cells’ by Mali et al. 2013 which achieved the display of synthetic zinc fingers on the surface of mammalian cells. This work was concerned with separating cells for cell labelling, tracking or for diagnostic purposes. We are adapting this idea by taking the perspective of bringing cells together rather than separating cells with the introduction of DNA structures that can hold together cells. | |
</p> | </p> | ||
Revision as of 20:53, 17 September 2015
