Difference between revisions of "Team:NYU-AD/Notebook/Week4"

 
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<p class="subtitle">Week 4</p>
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<h1>Week 4</h1>
 
<h2>26/08/15 </h2>
 
<h2>26/08/15 </h2>
 
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Latest revision as of 03:11, 18 September 2015

Week 4

26/08/15

  • Performed ligation of the promoter and tnaA
  • Amplifying the tnaA and tnaB sequence. .
  • Added 200ul of water (DNA- RNA free) into each tube
  • Performed the Nano Drop . The concentration of both tubes is approximately 7-8ug/ul.

27/08/2015

  • Nanodrop to verify concentration of resuspended DNA
  • Nuclease- free H2O used to resuspend the DNA was found to be contaminated with 4.8ug/ul of DNA
  • The actual DNA content of plasmids are around 6-8 ug/ul on average
  • We decided to proceed with the transformation since protocol specified 10ug- 100ug of DNA.

28/08/2015

  • We received the tnaa and the tnab tubes from IDT on the 26/08/15
  • Resuspended the tnaa and the tnab sequence by adding 200ul of water ( DNAse and RNAase free) into each tube.

29/08/2015

  • Nanodropped the DNA to verify the concentration of resuspended DNA. The Nuclease-free water that was used to reuspend the DNA was found to be contaminated with 4.8ng/ul of DNA. The actual DNA content of the plasmids is around 6-8ng/ul on average.
  • We still proceeded with the transformation of the DNA since the protocol specified 10ug-1000ug of DNA.