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| <h2>Experiments & Protocols</h2> | | <h2>Experiments & Protocols</h2> |
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− | <p>Describe the experiments, research and protocols you used in your iGEM project.</p> | + | <h4>Bacteria Media w/ agar (solid media)</h4> |
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| + | <h5>Materials</h5> |
| + | <li>500mL bottle</li> |
| + | <li>Deionized Water</li> |
| + | <li>Agar</li> |
| + | <li>LB Broth</li> |
| + | <li>Chloramphenicol</li> |
| + | <li>Yeast Extract</li> |
| + | <li>Bacto Peptone</li> |
| + | |
| + | <ol type=”1”> |
| + | <li>Add 250mL of deionized water to the 500mL bottle. </li> |
| + | <li>Weigh out 3.625g of agar using the weighing boat and add it to the 250mL of water.</li> |
| + | <li>Weigh out 5.0g of LB Broth using the weighing boat and add it to the 250mL of water.</li> |
| + | <li>Screw lid on bottle loosely.</li> |
| + | <li>Place autoclave tape on the lid of the bottle.</li> |
| + | <li>Place tray with approximately 1cm of water in height into the autoclave. </li> |
| + | <li>Place 500mL bottle into autoclave with "liquid" selected and press "start".</li> |
| + | <li>After an hour, remove bottle from autoclave. Let bottle sit for approximately 30 minutes.</li> |
| + | <li>Add 370uL of chloramphenicol to the 250mL solution.</li> |
| + | <li>Pour solution into petri dishes, covering the bottom of the dish.</li> |
| + | <li>Place two stripes on each dish.</li> |
| + | <li>Let dishes sit overnight.</li> |
| + | </ol> |
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| <h5>What should this page contain?</h5> | | <h5>What should this page contain?</h5> |