Difference between revisions of "Team:CU Boulder/Parts/team"

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<p><b>Genetic Switch for Bxb1 invertase</b></p>
 
<p><b>Genetic Switch for Bxb1 invertase</b></p>
 
<p>The Bxb1 invertase will invert the segment of DNA between the two recognition sites. In this case, Bxb1 will invert a terminator allowing for expression of a strong Anderson promoter.</p>
 
<p>The Bxb1 invertase will invert the segment of DNA between the two recognition sites. In this case, Bxb1 will invert a terminator allowing for expression of a strong Anderson promoter.</p>
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</div>
 
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Revision as of 02:15, 18 September 2015


<!DOCTYPE html> Team:CU_Boulder - 2015.igem.org

Team Parts



BBa_K1718001:

RFP under arabinose inducible pBad

This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.



BBa_K1718002:

Genetic Switch for Bxb1 invertase

The Bxb1 invertase will invert the segment of DNA between the two recognition sites. In this case, Bxb1 will invert a terminator allowing for expression of a strong Anderson promoter.



BBa_K1718003:

RFP under the LuxPr promoter

In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.



BBa_K1718004:

LuxI and GFP

LuxI leads to the production of AHL which, when in the presence of AHL, activates the LuxPr promoter. Downstream of the LuxI coding region is GFP, a reporter



BBa_K1718005:

Genetic switch for Bxb1 with LuxI and GFP

This part consists of the genetic switch described in BBa_K1718002 and the LuxI and GFP coding regions described in BBa_K1718004

Team:CU-Boulder - 2015.igem.org