Difference between revisions of "Team:Amoy/Notebook/Notebook"

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<div style="width: 80%; margin-left: 10%;">
 
<div style="width: 80%; margin-left: 10%;">
 
<p class="detail_h1">Purpose:</br></p>
 
<p class="detail_h1">Purpose:</br></p>
<p class="detail_p">make connection of isolated circuit with RBS_B0032 and gene_ldh</br></p>
+
<p class="detail_p">Ligation of isolated circuit with RBS_B0032 and gene_<i>leudh</i></br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_p">
 
<p class="detail_p">
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8. Extract the plasmids</br>
 
8. Extract the plasmids</br>
 
9. Electrophoresis analysis of plasmids</br></p>
 
9. Electrophoresis analysis of plasmids</br></p>
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
<p class="detail_p">isolated circuit with RBS_B0032 and gene_ldh</br></p>
+
<p class="detail_p">Isolated circuit with RBS_B0032 and gene_<i>leudh</i></br></p>
 
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</div>
 
</div>
 
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<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/c5/Amoy-Notebook_node13-4.jpeg" />
 
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/c/c5/Amoy-Notebook_node13-4.jpeg" />
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
<p class="detail_p">final circuit of RBS_B0030</br></br></br></p>
+
<p class="detail_p">Final circuit of RBS_B0030</br></br></br></p>
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 07:44, 18 September 2015

Aomy/Project

NOTEBOOK

Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are always destabilized in the isolation and purification process. What's more, the cofactor-NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH.

In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with rbs and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.

CONTACT US

Email: igemxmu@gmail.com

Website: 2015.igem.org/Team:Amoy

Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005