Difference between revisions of "Team:IISER Pune/Safety"

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<center><img src="https://static.igem.org/mediawiki/2015/1/17/Team_IISER_Pune_TT10.gif"></img></center><hr />
 
<center><img src="https://static.igem.org/mediawiki/2015/1/17/Team_IISER_Pune_TT10.gif"></img></center><hr />
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>The following safety guidelines were followed:</p>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<ul>
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<li>We used Escherichia coli (MG1655), a wild type strain which cannot survive outside laboratory conditions, to test all our constructs. </li>
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<li>Mycobacterium tuberculosis is a pathogenic strain. It falls under Risk Group 3 organisms and hence we will be using Mycobacterium smegmatis, a harmless non-pathogenic strain for our proof-of-concept experiments. </li>
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<li>Our device has termination sequence which causes cell death after the cells have reached a certain density</li>
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<li>We filled all the iGEM safety form.</li>
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</ul>
  
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<h4>Safe Project Design</h4>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
 
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<li>Choosing a non-pathogenic chassis</li>
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<li>All biological material were autoclaved before disposal.</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Fume hoods were used for corrosive reagents.</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Chemical and biological spills,if any, were contained and disposed depending on their nature.</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<li>Standard risk management procedure were followed based on the Institute’s policy.</li>
 
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</ul>
  
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{Team:Paris_Saclay/survey_badge|width=250px}
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<p> More details can be found at the Department of Biotechnology website: http://dbtbiosafety.nic.in/</p>
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<h4>Safe Lab Work</h4>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h4>Safe Shipment</h4>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 03:15, 19 September 2015


Top

The following safety guidelines were followed:

  • We used Escherichia coli (MG1655), a wild type strain which cannot survive outside laboratory conditions, to test all our constructs.
  • Mycobacterium tuberculosis is a pathogenic strain. It falls under Risk Group 3 organisms and hence we will be using Mycobacterium smegmatis, a harmless non-pathogenic strain for our proof-of-concept experiments.
  • Our device has termination sequence which causes cell death after the cells have reached a certain density
  • We filled all the iGEM safety form.
  • All biological material were autoclaved before disposal.
  • Fume hoods were used for corrosive reagents.
  • Chemical and biological spills,if any, were contained and disposed depending on their nature.
  • Standard risk management procedure were followed based on the Institute’s policy.

More details can be found at the Department of Biotechnology website: http://dbtbiosafety.nic.in/