Difference between revisions of "Team:SZU China/Design"
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<h3>UAA Orthogonal genectical system：</h3> | <h3>UAA Orthogonal genectical system：</h3> | ||
<p><h4>1.What is UAA?</h4> | <p><h4>1.What is UAA?</h4> | ||
| − | + | UAA is short for Unnatural Amino Acids.In most organisms,there are 20 kinds of amino acids in DNA encoding,which form the vast majority of proteins in the present life.Part of the ancient bacteria (Archaea) and (eubacteria) can encoding selenium (selenocysteine) or the pyrrolysine.The amino acids which do not exist in the natural protein sequence are called UAA.The unnatural amino acids used in the system must be stable, and can be stable in the cell. | |
| − | If the unnatural amino acids were inserted into the proteins expressed by the genetic system of encoding,the new physical and chemical properties of the proteins will expand the space of protein operation.<br> | + | If the unnatural amino acids were inserted into the proteins expressed by the genetic system of encoding,the new physical and chemical properties of the proteins will expand the space of protein operation. |
| − | <h4>2.The UAA using in our system:Acetyllysine(ACK)</h4> | + | <br><h4>2.The UAA using in our system:Acetyllysine(ACK)</h4> |
| − | + | Acetyllysine(ACK) is one of unnatural amino acid and only corresponds to a special aminoacyl-tRNA(tRNA). It can be added to the protein by Semantic coding of termination codon(UAG).</p> | |
<img src="https://static.igem.org/mediawiki/2015/5/5b/SZU_China_Orthogonal_2.jpg"> | <img src="https://static.igem.org/mediawiki/2015/5/5b/SZU_China_Orthogonal_2.jpg"> | ||
</section> | </section> | ||
<section> | <section> | ||
| − | + | ||
<p><h4>3.Use UAA as a switch to start the translation of proteins:</h4> | <p><h4>3.Use UAA as a switch to start the translation of proteins:</h4> | ||
| − | |||
To transmit the genetic information of mRNAs to proteins,after aa-tRNAs were synthesized by natural amino acids and tRNA via aaRS,they entered, and then entered the protein or peptide.To expand the genetic code and to lead new amino acids into the biosynthetic system,we need to identify the unnatural orthogonal codon,the tRNA with blank codon,the aaRS which can load UAA onto the tRNA specifically,the orthogonal mRNA,which can identify and highly efficient orthogonal translation,appropriate ribosome-binding site (RBS). These components need to satisfy the following conditions: (1) the only code must be recognized by the new tRNA, and has a link-coupled aaRS; (2) the aaRS/tRNA combination must be highly specific for unnatural amino acids in the host cells; (3) aaRs/tRNA pairs must be orthogonal differentiating endogenous aaRS/tRNA pairs(That is not cross in using).</p> | To transmit the genetic information of mRNAs to proteins,after aa-tRNAs were synthesized by natural amino acids and tRNA via aaRS,they entered, and then entered the protein or peptide.To expand the genetic code and to lead new amino acids into the biosynthetic system,we need to identify the unnatural orthogonal codon,the tRNA with blank codon,the aaRS which can load UAA onto the tRNA specifically,the orthogonal mRNA,which can identify and highly efficient orthogonal translation,appropriate ribosome-binding site (RBS). These components need to satisfy the following conditions: (1) the only code must be recognized by the new tRNA, and has a link-coupled aaRS; (2) the aaRS/tRNA combination must be highly specific for unnatural amino acids in the host cells; (3) aaRs/tRNA pairs must be orthogonal differentiating endogenous aaRS/tRNA pairs(That is not cross in using).</p> | ||
</section> | </section> | ||
<section> | <section> | ||
| − | < | + | <p><h4>3.Use UAA as a switch to start the translation of proteins:</h4> |
<p>Delightful ascenders contrast prototype. Ligature jakob nielsen user measure. Ligature contrast glyph rule of thirds composition interstitial dribbble. Mental model typography urbanized balance. Resolution rounded corners IDEO constraints dribbble persona. Portfolio sketch baseline 66-character line. Usability testing mental model simplicity aspect ratio pencil type. Usable sans-serif visualization leading prägnanz baseline pencil fireworks clarity omnigraffle.</p> | <p>Delightful ascenders contrast prototype. Ligature jakob nielsen user measure. Ligature contrast glyph rule of thirds composition interstitial dribbble. Mental model typography urbanized balance. Resolution rounded corners IDEO constraints dribbble persona. Portfolio sketch baseline 66-character line. Usability testing mental model simplicity aspect ratio pencil type. Usable sans-serif visualization leading prägnanz baseline pencil fireworks clarity omnigraffle.</p> | ||
<p>Paper prototype urbanized headroom typography splash screen pencil modal branding. Retina omnigraffle objectified descender navigation adobe ethnography. Innovate design by committee modern hero message. Contrast user-centered color theory keep it simple visuals bevel adobe descender splash screen. From focus group accessibility sans-serif archetype pixel-perfect complementary skeuomorphism. Focus group iconography figure-ground navigation user-centered omnigraffle from.</p> | <p>Paper prototype urbanized headroom typography splash screen pencil modal branding. Retina omnigraffle objectified descender navigation adobe ethnography. Innovate design by committee modern hero message. Contrast user-centered color theory keep it simple visuals bevel adobe descender splash screen. From focus group accessibility sans-serif archetype pixel-perfect complementary skeuomorphism. Focus group iconography figure-ground navigation user-centered omnigraffle from.</p> | ||
Revision as of 10:34, 18 September 2015
AND Gate
Orthogonal System
Orthogonal system:
Parallel and independent orthogonal system is one of the foundations of synthetic biology. This system has no or little cross with the natural biological system which is composed of unnatural base pair,shift code,unnatural amino acid,orthogonal aaRS(aminoacyl tRNA synthetase), orthogonal RNA synthetase,promoter and the orthogonal ribosome.These components work corporately or separately in the organism, programming cells with new functions, and also providing new techniques for researchers.
UAA Orthogonal genectical system：
1.What is UAA?
UAA is short for Unnatural Amino Acids.In most organisms,there are 20 kinds of amino acids in DNA encoding,which form the vast majority of proteins in the present life.Part of the ancient bacteria (Archaea) and (eubacteria) can encoding selenium (selenocysteine) or the pyrrolysine.The amino acids which do not exist in the natural protein sequence are called UAA.The unnatural amino acids used in the system must be stable, and can be stable in the cell. If the unnatural amino acids were inserted into the proteins expressed by the genetic system of encoding,the new physical and chemical properties of the proteins will expand the space of protein operation.2.The UAA using in our system:Acetyllysine(ACK)
Acetyllysine(ACK) is one of unnatural amino acid and only corresponds to a special aminoacyl-tRNA(tRNA). It can be added to the protein by Semantic coding of termination codon(UAG).
3.Use UAA as a switch to start the translation of proteins:
To transmit the genetic information of mRNAs to proteins,after aa-tRNAs were synthesized by natural amino acids and tRNA via aaRS,they entered, and then entered the protein or peptide.To expand the genetic code and to lead new amino acids into the biosynthetic system,we need to identify the unnatural orthogonal codon,the tRNA with blank codon,the aaRS which can load UAA onto the tRNA specifically,the orthogonal mRNA,which can identify and highly efficient orthogonal translation,appropriate ribosome-binding site (RBS). These components need to satisfy the following conditions: (1) the only code must be recognized by the new tRNA, and has a link-coupled aaRS; (2) the aaRS/tRNA combination must be highly specific for unnatural amino acids in the host cells; (3) aaRs/tRNA pairs must be orthogonal differentiating endogenous aaRS/tRNA pairs(That is not cross in using).3.Use UAA as a switch to start the translation of proteins:
Delightful ascenders contrast prototype. Ligature jakob nielsen user measure. Ligature contrast glyph rule of thirds composition interstitial dribbble. Mental model typography urbanized balance. Resolution rounded corners IDEO constraints dribbble persona. Portfolio sketch baseline 66-character line. Usability testing mental model simplicity aspect ratio pencil type. Usable sans-serif visualization leading prägnanz baseline pencil fireworks clarity omnigraffle.
Paper prototype urbanized headroom typography splash screen pencil modal branding. Retina omnigraffle objectified descender navigation adobe ethnography. Innovate design by committee modern hero message. Contrast user-centered color theory keep it simple visuals bevel adobe descender splash screen. From focus group accessibility sans-serif archetype pixel-perfect complementary skeuomorphism. Focus group iconography figure-ground navigation user-centered omnigraffle from.
Typesetting
Typesetting artifact rounded corners eye tracking braindump drawer menu mockup dropdown measure. Jony ive eye tracking script monospace heuristic tabs sketches. Affordance eye tracking scenario usable ligature typesetting clarity responsive. Information architecture golden ratio drawer menu header affordance aspect ratio. Balsamiq slab serif IDEO cognitive dissonance kerning pixel pixel-perfect. Pixel rounded corners header workflow portfolio.
Fireworks mobile skeuomorphism sitemap. Workflow iconography interaction design pixel-perfect serif. Mental model monospace typeface behavior change bauhaus from usability testing. Color theory user experience paper prototype narrative palette serif gradient header. Oblique modal 66-character line sketch responsive portfolio. Comic sans fireworks prägnanz monospace gradient design language jakob nielsen. Figure-ground pixel aspect ratio sketches rounded corners jony ive constraints mental model splash screen.
Placeholder text by Designer Ipsum.
Profile images are licensed under a Creative Commons BY-NC-SA 2.0 license. The images are from Greg Peverill-Conti's 1,000 faces project.
Web Design
Scenario archetype complementary responsive script pixel sidebar sitemap keep it simple. Complementary visuals footer CSS from alan cooper delightful. Photoshop iconography simplicity user experience affordance narrative ascenders contour. Slab serif interstitial skeuomorphism illustrator design by committee simplicity alan cooper eye tracking. Typography contrast mental model typesetting affordance narrative from CSS. Retina simplicity design by committee typography oblique.
Delightful ascenders contrast prototype. Ligature jakob nielsen user measure. Ligature contrast glyph rule of thirds composition interstitial dribbble. Mental model typography urbanized balance. Resolution rounded corners IDEO constraints dribbble persona. Portfolio sketch baseline 66-character line. Usability testing mental model simplicity aspect ratio pencil type. Usable sans-serif visualization leading prägnanz baseline pencil fireworks clarity omnigraffle.
Responsive
Card-sorting dropdown constraints alan cooper prägnanz iconography. Stakeholder balsamiq italic vector composition ethnography CSS monospace hierarchy. Eye tracking serif persona focus group.
Typesetting artifact rounded corners eye tracking braindump drawer menu mockup dropdown measure. Jony ive eye tracking script monospace heuristic tabs sketches. Affordance eye tracking scenario usable ligature typesetting clarity responsive. Information architecture golden ratio drawer menu header affordance aspect ratio. Balsamiq slab serif IDEO cognitive dissonance kerning pixel pixel-perfect. Pixel rounded corners header workflow portfolio.
Scenario navigation complementary jony ive helvetica archetype interstitial serif front-end. Constraints pencil usability gestalt design language mockup usability testing affordance jakob nielsen.
Prototype
Delightful ascenders contrast prototype. Ligature jakob nielsen user measure. Ligature contrast glyph rule of thirds composition interstitial dribbble. Mental model typography urbanized balance. Resolution rounded corners IDEO constraints dribbble persona. Portfolio sketch baseline 66-character line. Usability testing mental model simplicity aspect ratio pencil type. Usable sans-serif visualization leading prägnanz baseline pencil fireworks clarity omnigraffle.
Paper prototype urbanized headroom typography splash screen pencil modal branding. Retina omnigraffle objectified descender navigation adobe ethnography. Innovate design by committee modern hero message. Contrast user-centered color theory keep it simple visuals bevel adobe descender splash screen. From focus group accessibility sans-serif archetype pixel-perfect complementary skeuomorphism. Focus group iconography figure-ground navigation user-centered omnigraffle from.
Typography
Typesetting artifact rounded corners eye tracking braindump drawer menu mockup dropdown measure. Jony ive eye tracking script monospace heuristic tabs sketches. Affordance eye tracking scenario usable ligature typesetting clarity responsive. Information architecture golden ratio drawer menu header affordance aspect ratio. Balsamiq slab serif IDEO cognitive dissonance kerning pixel pixel-perfect. Pixel rounded corners header workflow portfolio.
Fireworks mobile skeuomorphism sitemap. Workflow iconography interaction design pixel-perfect serif. Mental model monospace typeface behavior change bauhaus from usability testing. Color theory user experience paper prototype narrative palette serif gradient header. Oblique modal 66-character line sketch responsive portfolio. Comic sans fireworks prägnanz monospace gradient design language jakob nielsen. Figure-ground pixel aspect ratio sketches rounded corners jony ive constraints mental model splash screen.
Placeholder text by Designer Ipsum.
Profile images are licensed under a Creative Commons BY-NC-SA 2.0 license. The images are from Greg Peverill-Conti's 1,000 faces project.