Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"

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<p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.  
 
<p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.  
 
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
 
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
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<h2>5. BBa_K1603004: Ssb</h2>
 
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<p>From Deinococcus Radiodurans.
 
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Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
 
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</p>
 

Revision as of 12:31, 18 September 2015



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BioBricks

1. BBa_K1603000: STE2MAM2

Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.

2. BBa_K1603001: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.

3. BBa_K1603002: pTPI1

Promoter to TPI1.

4. BBa_K1603003: RecA

Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.