|
|
Line 15: |
Line 15: |
| <ul> | | <ul> |
| <li><a href="https://2015.igem.org/Team:UChile-OpenBio">HOME</a></li> | | <li><a href="https://2015.igem.org/Team:UChile-OpenBio">HOME</a></li> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Team">TEAM</a></li> | + | <li> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Description">PROJECT</a></li> | + | <a href="https://2015.igem.org/Team:UChile-OpenBio/Description">PROJECT</a> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Experiments">EXPERIMENT</a></li> | + | <ul> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Description">PROJECT DESCRIPTION</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Experiments">EXPERIMENT AND PROTOCOLS</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Results">RESULTS</a></li> |
| + | </ul> |
| + | </li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Notebook">NOTEBOOK</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Atributions">ATRIBUTIONS</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Collaborations">COLLABORATION</a></li> |
| <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Practices">HUMAN PRACTICES</a></li> | | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Practices">HUMAN PRACTICES</a></li> |
− | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Collaborations">COLLABORATIONS</a></li> | + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Safety">SAFETY</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Modeling">MODELING</a></li> |
| + | <li><a href="https://2015.igem.org/Team:UChile-OpenBio/Team">TEAM</a></li> |
| </ul> | | </ul> |
| </nav> | | </nav> |
Line 29: |
Line 39: |
| <html> | | <html> |
| <!-- SI EXPORT END --> | | <!-- SI EXPORT END --> |
− | <section class="collaborations"> | + | <!-- ANTIGUA COLLABORATIONS --> |
− | <span class="titulo_seccion">Collaboration</span> | + | <section class="atributions"> |
| + | <span class="titulo_seccion">Atributions</span> |
| <img id="activities_title" class="" alt="Activities" src="https://static.igem.org/mediawiki/2015/0/05/Activities_title.png" /> | | <img id="activities_title" class="" alt="Activities" src="https://static.igem.org/mediawiki/2015/0/05/Activities_title.png" /> |
| <article> | | <article> |
Line 36: |
Line 47: |
| <div class="half"> | | <div class="half"> |
| <div class="item_actividad"> | | <div class="item_actividad"> |
− | <!-- Cambio de posicion december -->
| + | <div class="item_actividad-titulo"><span>January 2015:</span><span>Conception Idea</span></div> |
− | <!-- cambio el contenido general de la pagina-->
| + | |
− | | + | |
− | <!-- Team Formation-->
| + | |
− | | + | |
− | <div class="item_actividad-titulo"><span>December 2014:</span><span>Team Formation</span></div> | + | |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p>At that time there was an organized group from <b> <span style="color:#CC0000"> Universidad de Chile </span></b> called <b> <span style="color:#CC0000"> OpenBio </span></b>. That team was funded on December 2013 by <b> <span style="color:#CC0000"> Luis Rodriguez </span></b>and <b><span style="color:#CC0000">Pablo Troncoso</span></b> (we miss you!). Organization’s main objectives are:<br/>
| + | <img class="" alt="Conception Idea" src="https://static.igem.org/mediawiki/2015/3/32/Collaboration_conception_idea.png" /> |
− | 1.- Boosting Synthetic Biology in Chile. <br/>
| + | |
− | 2.- Development of low cost Biotechnologies machines.<br/>
| + | |
− | 3.- Periodically attendance to the iGEM Competition.<br/>
| + | |
− | The group used to participate in different activities in university to spread synthetic biology through workshops and informative talks.</p>
| + | |
− | <img class="" alt="Team Formation1" src="https://static.igem.org/mediawiki/2015/b/b1/Team_formation_2.png" /> | + | |
− | <p>Thanks to the activities that the group developed and others that were attended by them, was possible to contact with people interested in being part of the project and very committed to the tasks involved in developing it.</p>
| + | |
− | <img class="" alt="Team Formation2" src="https://static.igem.org/mediawiki/2015/6/60/Team_formation.png" />
| + | |
− | <p> Finally we formed the Team iGEM Uchile-OpenBio, we all feel part of the team and if you want to know more about the members of the team we invite you to see our <font size="42"> <a href="https://2015.igem.org/Team:UChile-OpenBio/Team" >Team Description</a></p>
| + | |
| </div> | | </div> |
| </div> | | </div> |
− |
| |
− | <!--Team Registration-->
| |
− |
| |
| <div class="item_actividad"> | | <div class="item_actividad"> |
− | <div class="item_actividad-titulo"><span>March 2015:</span><span>Team Registration</span></div> | + | <div class="item_actividad-titulo"><span>April 2015:</span><span>Team Consolidation</span></div> |
− | <div class="item_actividad-cuerpo"> | + | <div class="item_actividad-cuerpo"> |
− | | + | <p>We have achieved from a multidisciplinary team for our project. The team is composed by Engineering students, Industrial Designer and Journalist.</p> |
− | <p>The group inscription fee was paid by <b><span style="color:#660066">CeBiB </b> </span> and <b><span style="color:#660066">DIQBT</b> </span> for a total amount of | + | <img class="" alt="Team Consolidation" src="https://static.igem.org/mediawiki/2015/3/3d/Collaboration_team_consolidation.png" /> |
− | <span style="color:#660066"><b>4000 USD</span></b>. CeBiB also lent us their laboratories to developthe project and help us with diffusion. <br/>
| + | |
− | <b><span style="color:#660066"> Centre for Biotechnology and Bioengineering (CeBiB) </span></b> is a center of excellence in frontier research by an interdisciplinary team composed of renowned national and international, able to investigate key issues of modern biotechnology and bioengineering, including important applications in industry.</p>
| + | |
− | <img class="" alt="Team Registration CeBiB" src="https://static.igem.org/mediawiki/2015/9/98/Collaboration_cebib.png" /><br> | + | |
− | <p> <b> <span style="color:#660066"> Department of Chemical Engineering and Biotechnology (DIQBT) </span></b> is part of Faculty of phisics sciences and mathematics (FCFM), Univerisdad de Chile. It teaches two engineering specialities: Chemical and Biotechnological Engineering.
| + | |
− | <img class="" alt="Team Registration DIQBT" src="https://static.igem.org/mediawiki/2015/2/23/Collaboration_diqbt.png" />
| + | |
− | | + | |
− | <p><span style="color:#660066"> <b> ¿How did we obtain this support at the first time? </span> </b> <br/>
| + | |
− | <b> <span style="color:#660066"> Ignacio Ardiles </span></b> and <b><span style="color:#660066"> Luis Rodriguez </span></b> created a Sponsorship letter offering to the institutions being our sponsors. In exchange for their support we offer them: <BR/>
| + | |
− | 1.- Team official clothing.<BR/>
| + | |
− | 2.- Our official Website and social networks.<BR/>
| + | |
− | 3.- 10 cm downside the official poster.<BR/>
| + | |
− | 4.- Our Wiki project, actually this part.<BR/>
| + | |
− | Also we will honor our sponsors at the end of our presentation with special mentions. The sponsorship letter is attached at the following <a href="https://2015.igem.org/File:Uchile-openbio_OB_sponsors.pdf">link. </a></p>
| + | |
| </div> | | </div> |
| </div> | | </div> |
− |
| |
− | <!------Team Consolidation------>
| |
− |
| |
| <div class="item_actividad"> | | <div class="item_actividad"> |
− | <div class="item_actividad-titulo"><span>April 2015:</span><span>Team Consolidation</span></div> | + | <div class="item_actividad-titulo"><span>June 2015:</span><span>Because We ́re happy! PRIZE</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p> After the registration the team set up weekly meetings to plan the work of each week. Also each member of the team has adopted a project area. The distribution of areas was agreed by all members. <br/> | + | <p>We were announced that UChile OpenBio got 2° Award Competition for Innovative Ideas & Technology Copec-UC Foundation and StgoLabSpace. The award generated significant project visibility.</p> |
− | We agreed that the responsible of each area will coordinate all the activities of that specific area. However, all team members are responsible for asisting in the areas applying for aid.<br/> | + | <img class="" alt="Because We ́re happy! PRIZE" src="https://static.igem.org/mediawiki/2015/3/32/Collaboration_prize.png" /> |
− | Each area has to report their advance and show their main activities for next weeks. With this assigment we are training our leadership and planning skills on each member.<br/>
| + | |
− | <b><span style="color:#CC0000"> Project areas and responsibles</span></b></p><br/>
| + | |
− | <img class="" alt="Team Consolidation" src="https://static.igem.org/mediawiki/2015/a/a5/Table.png" /> | + | |
| </div> | | </div> |
| </div> | | </div> |
− | | + | <div class="item_actividad morado"> |
− | <!-- We are happy!-->
| + | <div class="item_actividad-titulo"><span>June 2015:</span><span>SIGMA-ALDRICH</span></div> |
− | | + | |
− | <div class="item_actividad"> | + | |
− | <div class="item_actividad-titulo"><span>June 2015:</span><span>Because We ´re happy! Price</span></div> | + | |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <iframe src="https://player.vimeo.com/video/139687398" width="500" height="288" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>
| + | <p>Sigma-Aldrich provided us for the laboratory reagents around USD $1900 (Golden Sponsor).<br/> |
− | <p>We received an invitation for a contest conducted by <b><span style="color:#CC0000"> Aplica tu Idea</span></b> and <b><span style="color:#CC0000"> Fundacion COPEC UC</span></b> where you can give a short pitch on 3 minutes to present your idea. The first place wins $ 200 to support the project. Despite the price was less than 1% of our budget it was a great oportunity to show our project and get prepaired to next presentations. <br> | + | It was very important to decrease the time to providing reagents for project.<br/> |
− | <b><span style="color:#CC0000"> Gustavo Calvo</span></b> was in charge of creating the speech and make the presentation at the headquarters of the Universidad Católica. He obtained the <b><span style="color:#CC0000"> Second place</span></b> on the contest and was awarded with a special price for the team. Although we did not get 200 dollars, the contest opened our way to make the project better known. </p>
| + | Attach archive Sponsorship Invoice.</p> |
− | <img class="" alt="Because We ´re happy! PRIZE" src="https://static.igem.org/mediawiki/2015/3/32/Collaboration_prize.png" /> | + | <img class="" alt="SIGMA-ALDRICH" src="https://static.igem.org/mediawiki/2015/f/f5/Collaboration_sigma.jpg" /> |
| </div> | | </div> |
| </div> | | </div> |
− |
| |
− | <!-- gBlocks arrived-->
| |
− |
| |
− |
| |
| <div class="item_actividad"> | | <div class="item_actividad"> |
− | <div class="item_actividad-titulo"><span>July 2015:</span><span>gBlocks arrived</span></div> | + | <div class="item_actividad-titulo"><span>July 2015:</span><span>gBlocks ARRIVED</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p><b><span style="color:#CC0000"> gBlocks </span> </b> were provided by <span style="color:#CC0000"><b> Integrated DNA Technologies</span> </b>. The arrivals means to us that we could finally began to assemble our genetic circuit. </p> | + | <p>The gBlocks are pieces of DNA around 500 pb long, so if you have gens of around 1Kb, you will have to link two gBlocks. For link gBocks, we use the Gibson Assembly technique.<br/> |
− | <br/>
| + | The gBlocks arrived means to us that we could finally began to assemble our genetic circuit.</p> |
− | <img class="" alt="gBlocks ARRIVED" src="https://static.igem.org/mediawiki/2015/c/c0/Collaboration_gblocks.png" /> <br> | + | <img class="" alt="gBlocks ARRIVED" src="https://static.igem.org/mediawiki/2015/c/c0/Collaboration_gblocks.png" /> |
− | | + | |
− | <p> <b><span style="color:#CC0000">But... Why are important Gblocks? </span></b> <br>
| + | |
− | | + | |
− | For every design in synthetic biology, a high fidelity technique is imperative to obtain large fragments of DNA for coding our gene of interest with the regulation elements. The group initially considered obtain the genetic source from the microorgansisms, amplify them and then produce the assemblies by overlap extension PCR. Thanks to gBlocks we could save all this work in laboratory that would require weeks, shipping times of biological material and we would not be able perform codon optimization </p>
| + | |
− | <img class="" alt="IDTlogo" src="https://static.igem.org/mediawiki/2015/b/ba/Idt_logo2015.jpg" />
| + | |
| </div> | | </div> |
| </div> | | </div> |
− |
| |
− |
| |
− | <!--Open Lab -->
| |
− |
| |
| <div class="item_actividad"> | | <div class="item_actividad"> |
− | <div class="item_actividad-titulo"><span>July 2015:</span><span>Open Beauchef, FCFM</span></div> | + | <div class="item_actividad-titulo"><span>July 2015:</span><span>OpenLab FCFM</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <p><b><span style="color:#CC0000">Open Beauchef </span></b>consists of a co-working space (collaborative work) that FCFM facilities. It is located at 851 Avenue Beauchef in the OpenLab. It is designed to create a meeting place for cooperative activities that allow interaction between various innovation actors. It includes students, researchers and developers from every faculty of the Universidad de Chile, together with participants from academia, business, public sector, graduates and investors.</p> | + | <p>We participate of a sessions for guide and empower our project.<br/> |
− | <img class="" alt="OpenLab FCFM0" src="https://static.igem.org/mediawiki/2015/2/25/Uchile-Openbio_OpenBeauchef.png" />
| + | The development of entrepreneurial skills in the team was supported of OpenLab Beauchef. We participate in workshops innovation and entrepreneurship. It was a strategic stakeholder for the team.</p> |
− | | + | <img class="" alt="OpenLab FCFM" src="https://static.igem.org/mediawiki/2015/7/7d/Collaboration_pizarra.jpg" /> |
− | <p> The contact was made first by <b><span style="color:#CC0000">Severine Cazaux </span></b>, who tried to contact our Dean Faculty. At first he conducted us to the OpenLab to obtain some team support. They helped us with <b><span style="color:#CC0000">Mentoring </span></b> through 2 workshops where we could see our team interests and availability for the project. They also gave us some tasks to gain support on the project: We had to collect 4 interests letters related to our project. <br/>
| + | |
− | | + | |
− | After that and other meetings they wrote a report of the team with the results of the activities. Finally they gave us tips for our team work.<br/>
| + | |
− | | + | |
− | It realy helped us to align all members interests. Also it was our first approachment to our Faculty.<br/></p> | + | |
− | | + | |
− | <img class="" alt="OpenLab FCFM1" src="https://static.igem.org/mediawiki/2015/7/7d/Collaboration_pizarra.jpg" /> | + | |
| </div> | | </div> |
| </div> | | </div> |
− |
| |
− | <!-- Diario Atacama-->
| |
| <div class="item_actividad rosado"> | | <div class="item_actividad rosado"> |
| <div class="item_actividad-titulo"><span>July 2015:</span><span>El Diario de Atacama</span></div> | | <div class="item_actividad-titulo"><span>July 2015:</span><span>El Diario de Atacama</span></div> |
Line 186: |
Line 137: |
| </div> | | </div> |
| </div> | | </div> |
− |
| |
− | <!--Mitad restante -->
| |
− |
| |
| <div class="half last"> | | <div class="half last"> |
− | <div class="item_actividad">
| + | <div class="item_actividad"> |
− | <div class="item_actividad-titulo"><span>January 2015:</span><span>Conception Idea</span></div> | + | <div class="item_actividad-titulo"><span>December 2014:</span><span>Team Formation</span></div> |
| <div class="item_actividad-cuerpo"> | | <div class="item_actividad-cuerpo"> |
− | <img class="" alt="Conception Idea" src="https://static.igem.org/mediawiki/2015/3/32/Collaboration_conception_idea.png" /> | + | <p>We started the formation of our multidisciplinary iGEM team to make possible our project idea of a open source club “OpenBio”; however, at the beginning, we were a team of only Biotechnology Engineering. Our mission: To boost Synthetic Biology for new generations in Chile, Open Source way and non-profit. Our first challenge accepted was to have our first iGEM Team.</p> |
| + | <img class="" alt="Team Formation" src="https://static.igem.org/mediawiki/2015/1/14/Collaboration_team_formation.png" /> |
| + | </div> |
| + | </div> |
| + | <div class="item_actividad"> |
| + | <div class="item_actividad-titulo"><span>March 2015:</span><span>Team Registration</span></div> |
| + | <div class="item_actividad-cuerpo"> |
| + | <p>The registration was paid by CeBiB and DIQBT. They also provide their laboratories and spaces to develop the project.</p> |
| + | <img class="" alt="Team Registration CeBiB" src="https://static.igem.org/mediawiki/2015/9/98/Collaboration_cebib.png" /><br> |
| + | <img class="" alt="Team Registration DIQBT" src="https://static.igem.org/mediawiki/2015/2/23/Collaboration_diqbt.png" /> |
| </div> | | </div> |
| </div> | | </div> |
Line 208: |
Line 165: |
| <p><em>“Silence is the element in which all great things are formed”</em><br>Thomas Carlyle (1795-1881)</p> | | <p><em>“Silence is the element in which all great things are formed”</em><br>Thomas Carlyle (1795-1881)</p> |
| <p id="video"><iframe src="https://player.vimeo.com/video/130371509?color=ffffff&title=0&byline=0&portrait=0" width="420" height="236" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <p></p></p> | | <p id="video"><iframe src="https://player.vimeo.com/video/130371509?color=ffffff&title=0&byline=0&portrait=0" width="420" height="236" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <p></p></p> |
− | </div>
| |
− | </div>
| |
− | <!-- Correccion orden linea de tiempo -->
| |
− | <div class="item_actividad morado">
| |
− | <div class="item_actividad-titulo"><span>June 2015:</span><span>SIGMA-ALDRICH</span></div>
| |
− | <div class="item_actividad-cuerpo">
| |
− | <p>Sigma-Aldrich provided us for the laboratory reagents around USD $1900 (Golden Sponsor).<br/>
| |
− | It was very important to decrease the time to providing reagents for project.<br/>
| |
− | Attach archive Sponsorship Invoice.</p>
| |
− | <img class="" alt="SIGMA-ALDRICH" src="https://static.igem.org/mediawiki/2015/f/f5/Collaboration_sigma.jpg" />
| |
| </div> | | </div> |
| </div> | | </div> |