Difference between revisions of "Team:IIT Delhi/modelling"

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<img style="margin-left:50%;height:100px;width:500px;" src="https://static.igem.org/mediawiki/2015/8/8f/Imagem3_iitd.png" alt="equation1"><br/>
 
<img style="margin-left:50%;height:100px;width:500px;" src="https://static.igem.org/mediawiki/2015/8/8f/Imagem3_iitd.png" alt="equation1"><br/>
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<p>The rest of the reactions in this pathway are assumed to proceed via Henri-Michaelis-Menten kinetics: <br/>
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<img style="margin-left:50%;" src="https://static.igem.org/mediawiki/2015/d/d9/IITDmodellingeq2.png">
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<br/>
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</p>
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<table>
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  <tr>
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    <th>Enzyme</th>
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    <th>Km (micromol/l)</th>
 +
  </tr>
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  <tr>
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    <td>Uroporphyrinogen decarboxylase ( hemE)</td>
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    <td>6.0</td>
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  <tr/>
 +
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  <tr>
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    <td>Coproporphyrinogen iii dehydrogenase</td>
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    <td>210</td>
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  <tr/>
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  <tr>
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    <td>Protoporphyrinogen dehydrogenase (HemG) </td>
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    <td>7</td>
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  <tr/>
 +
 +
  <tr>
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    <td>Ferrochelatase(hemH) </td>
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    <td>4.7</td>
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  <tr/>
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  <tr>
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    <td>Glutamate-tRNA synthase</td>
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    <td>1.9</td>
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  <tr/>
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 +
  <tr>
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    <td>Glutamate-1-semialdehyde 2,1-aminomutase</td>
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    <td>46</td>
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  <tr/>
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  <tr>
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    <td>Porphobilinogen synthase</td>
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    <td>800</td>
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  <tr/>
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</table>
  
 
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Revision as of 12:36, 18 September 2015

Introduction

Three of the proteins required in the project are cytochrome c proteins (haem proteins). Hence, the heme biosynthesis pathway needs to be modelled. Pathway of heme production in E. coli (C-5 pathway from glutamate)

Mathematical model

Pathway of heme production in E. coli (C-5 pathway from glutamate):
Also, d-Aminolevulinate (ALA) produced as an intermediate in the above reactions. -Aminolevulinate Synthase (ALA Synthase) is the committed step of the heme synthesis pathway, and is usually rate-limiting for the overall pathway. Regulation occurs through control of gene transcription. Heme functions as a feedback inhibitor, repressing transcription of the gene for -Aminolevulinate Synthase.
A non-competitive irreversible feedback inhibition model is assumed for this step with a Ki value of 0.02 mM. Hence, the following equation governs the production of ALA:



equation1

The rest of the reactions in this pathway are assumed to proceed via Henri-Michaelis-Menten kinetics:

Enzyme Km (micromol/l)
Uroporphyrinogen decarboxylase ( hemE) 6.0
Coproporphyrinogen iii dehydrogenase 210
Protoporphyrinogen dehydrogenase (HemG) 7
Ferrochelatase(hemH) 4.7
Glutamate-tRNA synthase 1.9
Glutamate-1-semialdehyde 2,1-aminomutase 46
Porphobilinogen synthase 800

A non-competitive irreversible feedback inhibition model is assumed for this step with a ki value of 0.02 mM. Hence, the following equation governs the conversion to ALA:

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