Difference between revisions of "Team:KU Leuven/Research/Basic Part"

Line 129: Line 129:
 
</div>
 
</div>
 
</br>
 
</br>
 
+
</div>
  
 
</div>
 
</div>

Revision as of 13:07, 18 September 2015

Basics Parts

On this page you can find all our basic parts needed for the system to work. To learn more about them, click the titles below!

Name Construct Length Description
BBa_K1709002 CheZ-GFP 1401 bp text
BBa_K1709000 LuxI-His 609 bp text
BBa_K1709004 LuxR-E 835 bp text

CheZ-GFP


LuxI-His

Theory


Motility Test Assay

Protocol

1. Prepare selective media (LB with 0.25% agar (2,5 g/L) in Petri dishes (85 mm dia).
2. Apply 1.5 µL of the diluted cell suspensions from mid-log-phase cultures (~2×105 cells/µL (OD=0.5)) to the center of the plates, and let dry in air for 15 min.
3. Incubate at 37 °C for 10 h.

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be