Difference between revisions of "Team:Czech Republic/Parts"
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Several Basic parts were constructed during the summer. We contributed to the Registry with 8 different basic parts, ready to use in ''Saccharomyces cerevisiae''. Function of all these parts was experimentally validated. | Several Basic parts were constructed during the summer. We contributed to the Registry with 8 different basic parts, ready to use in ''Saccharomyces cerevisiae''. Function of all these parts was experimentally validated. | ||
Revision as of 17:56, 18 September 2015
Parts
Description
Basic Parts
Several Basic parts were constructed during the summer. We contributed to the Registry with 8 different basic parts, ready to use in Saccharomyces cerevisiae. Function of all these parts was experimentally validated.
We sent 4 different mating pheromones:
- Mating pheromone (S. cerevisiae) - [http://parts.igem.org/Part:BBa_K1690000 BBa_K1690000]
- Mating pheromone (C. albicans) - [http://parts.igem.org/Part:BBa_K1690001 BBa_K1690001]
- Mating pheromone (C. tropicalis) - [http://parts.igem.org/Part:BBa_K1690002 BBa_K1690002]
- Mating pheromone (L. elongisporus) - [http://parts.igem.org/Part:BBa_K1690003 BBa_K1690003]
And with the sequences of 2 different pheromone receptors:
- Pheromone receptor (C. albicans) - [http://parts.igem.org/Part:BBa_K1690004 BBa_K1690004]
- Pheromone receptor (C. parapsilosis) - [http://parts.igem.org/Part:BBa_K1690005 BBa_K1690005]
The BioBricks above were constructed by Module 2, where you can find details of their construction and function.
Also, 3 different reporters ready to use in Saccharomyces were prepared and sent:
- pADH1_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690006 BBa_K1690006]
- pCYC1v3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690007 BBa_K1690007]
- pTv3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690008 BBa_K1690008]
These BioBricks were constructed by Module 1, where you can find details of their construction and function.
Part Collection
Our submitted parts can be divided into two separate collections:
Signals & Receptors
- Mating pheromone (S. cerevisiae) - [http://parts.igem.org/Part:BBa_K1690000 BBa_K1690000]
- Mating pheromone (C. albicans) - [http://parts.igem.org/Part:BBa_K1690001 BBa_K1690001]
- Mating pheromone (C. tropicalis) - [http://parts.igem.org/Part:BBa_K1690002 BBa_K1690002]
- Mating pheromone (L. elongisporus) - [http://parts.igem.org/Part:BBa_K1690003 BBa_K1690003]
- Pheromone receptor (C. albicans) - [http://parts.igem.org/Part:BBa_K1690004 BBa_K1690004]
- Pheromone receptor (C. parapsilosis) - [http://parts.igem.org/Part:BBa_K1690005 BBa_K1690005]
Yeast reporters
- pADH1_GFP (Yeast reporter) - [http
- //parts.igem.org/Part:BBa_K1690006 BBa_K1690006] : GFP under the control of constitutive pADH1 promoter
- pCYC1v3_GFP (Yeast reporter) - [http
- //parts.igem.org/Part:BBa_K1690007 BBa_K1690007] : GFP under the control of constitutive pCYC1v3 promoter
- pTv3_GFP (Yeast reporter) - [http
- //parts.igem.org/Part:BBa_K1690008 BBa_K1690008] : GFP under the control of repressible pTet promoter