Difference between revisions of "Team:Pasteur Paris/Week 10"
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<li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5mol/L</li></br> | <li style="text-align:left">Creation of a stock solution of soluble TPA in water at 0,5mol/L</li></br> | ||
<li style="text-align:left">Test of the TPA Solubilization in LB Broth</li></br> | <li style="text-align:left">Test of the TPA Solubilization in LB Broth</li></br> | ||
− | <li style="text-align:left"><u>Preparation of different media containing TPA and EG:</u></br>Media 1 to 10 are made in solid form:</li> | + | <li style="text-align:left"><u>Preparation of different media containing (TPA) terephthalic acid and (EG) ethylene glycol:</u></br>Media 1 to 10 are made in solid form:</li> |
<ol> | <ol> | ||
<li style="text-align:left">M9</li> | <li style="text-align:left">M9</li> | ||
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</ol> | </ol> | ||
<li style="text-align:left">Media 11 to 15 are made in both solid and liquid form:</li> | <li style="text-align:left">Media 11 to 15 are made in both solid and liquid form:</li> | ||
− | + | <ol> | |
+ | <li style="text-align:left">M9</li> | ||
+ | <li style="text-align:left">M9 + TPA (1.2mM)</li> | ||
+ | <li style="text-align:left">M9 + TPA (6mM)</li> | ||
+ | <li style="text-align:left">M9 + TPA (12mM)</li> | ||
+ | <li style="text-align:left">M9 + sucrose (1.2mM)</li> | ||
+ | <li style="text-align:left">M9 + sucrose (6mM)</li> | ||
+ | <li style="text-align:left">M9 + sucrose (12mM)</li> | ||
+ | <li style="text-align:left">M9 + sucrose + TPA (6mM)</li> | ||
+ | <li style="text-align:left">M9 + sucrose + TPA (1,2mM)</li> | ||
+ | </ol> | ||
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Revision as of 12:46, 18 September 2015
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Week 10 Toxicity and solubility of TPA (Terephthalic acid)
Enzymatic Assay of NB-EsteraseFigure 8: Gel for verification of ligation. There is no band for the ligation sample. Gibson Assembly
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