Difference between revisions of "Template:Team:TU Eindhoven/Equipment HTML"
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− | The MiniSpin Centrifuge is a powerful centrifuge that can accelerate up to twelve 1.5/2.0 ml Eppendorf Tubes to a maximum speed of 13,400 rpm (rcf: 12,100 x g). Balance is very important for this equipment. We used this centrifuge for several experiments, such as PCR Purification, Miniprepping, Gel Extraction and preparing samples for FACS. | + | The MiniSpin Centrifuge (figure 1A) is a powerful centrifuge that can accelerate up to twelve 1.5/2.0 ml Eppendorf Tubes to a maximum speed of 13,400 rpm (rcf: 12,100 x g). Balance is very important for this equipment. We used this centrifuge for several experiments, such as PCR Purification, Miniprepping, Gel Extraction and preparing samples for FACS. |
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<img class="left1" src="https://static.igem.org/mediawiki/2015/4/41/TU_Eindhoven_minispin.png"> | <img class="left1" src="https://static.igem.org/mediawiki/2015/4/41/TU_Eindhoven_minispin.png"> | ||
<img class="right1" src="https://static.igem.org/mediawiki/2015/f/fa/TU_Eindhoven_Tabletop.png"> | <img class="right1" src="https://static.igem.org/mediawiki/2015/f/fa/TU_Eindhoven_Tabletop.png"> | ||
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+ | Figure 1A: MiniSpin Centrifuge <br /> | ||
+ | Figure 1B: Tabletop Centrifuge <br /> | ||
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+ | Tabletop Centrifuge | ||
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+ | The Tabletop Centrifuge (figure 1B) can be used to centrifuge culture tubes. For this centrifuge, balance is also very important, just as with the MiniSpin Centrifuge. We used this centrifuge for several experiments, such as miniprepping. | ||
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+ | MJ Mini Thermal Cycler | ||
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+ | The compact, 48-well MJ Mini thermal cycler (figure 2) is a powerful thermal cycler that has been used to perform all kinds of PCR reaction, such as digestion and ligation. 0.2 ml PCR tubes can be used. Its thermal range is 0-99°C. This thermal cycler also offers a thermal gradient technology so that you can optimize reactions for maximum efficiency and accurate quantitation with a gradient range of 35-99°C. | ||
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+ | <img class="left2b" src="https://static.igem.org/mediawiki/2015/c/c9/TU_Eindhoven_PCR.png"> | ||
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+ | Figure 2: MJ Mini Thermal Cycler. | ||
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Revision as of 13:26, 18 September 2015
Lab Equipment
In the lab we used many devices to perform our experiments. Followed is a short description of each equipment.
MiniSpin Centrifuge
The MiniSpin Centrifuge (figure 1A) is a powerful centrifuge that can accelerate up to twelve 1.5/2.0 ml Eppendorf Tubes to a maximum speed of 13,400 rpm (rcf: 12,100 x g). Balance is very important for this equipment. We used this centrifuge for several experiments, such as PCR Purification, Miniprepping, Gel Extraction and preparing samples for FACS.
Tabletop Centrifuge
The Tabletop Centrifuge (figure 1B) can be used to centrifuge culture tubes. For this centrifuge, balance is also very important, just as with the MiniSpin Centrifuge. We used this centrifuge for several experiments, such as miniprepping.
MJ Mini Thermal Cycler
The compact, 48-well MJ Mini thermal cycler (figure 2) is a powerful thermal cycler that has been used to perform all kinds of PCR reaction, such as digestion and ligation. 0.2 ml PCR tubes can be used. Its thermal range is 0-99°C. This thermal cycler also offers a thermal gradient technology so that you can optimize reactions for maximum efficiency and accurate quantitation with a gradient range of 35-99°C.