Difference between revisions of "Team:Chalmers-Gothenburg/Project Results"
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<h1>Overview</h1> | <h1>Overview</h1> | ||
| + | <p>• Assembled and integrated two different safety switches, based on TPK2 overexpression or mRFP expression induced at low ATP levels, into CEN.PK2.</p> | ||
| + | <p>• SS TPK2 shows reduced growth rate after OD-measurement but no change in viability compared to wild type.</p> | ||
| + | <p>• mRFP measurements show that connection of pTEF1 to pSUC2 maintains the ATP repression mechanism of pSUC2, while achieving higher expression at low ATP levels.</p> | ||
| + | <p>• Successfully constructed and integrated the system to detect the P-factor from S.pombe into CEN.PK2.</p> | ||
| + | <p>• Fluorescent cells in DAS</p> | ||
| + | <p>• Amplified all parts of the PUR system, but obtained vector-only clones after Gibson and transformation into E.coli</p> | ||
Revision as of 16:30, 18 September 2015
Overview
• Assembled and integrated two different safety switches, based on TPK2 overexpression or mRFP expression induced at low ATP levels, into CEN.PK2.
• SS TPK2 shows reduced growth rate after OD-measurement but no change in viability compared to wild type.
• mRFP measurements show that connection of pTEF1 to pSUC2 maintains the ATP repression mechanism of pSUC2, while achieving higher expression at low ATP levels.
• Successfully constructed and integrated the system to detect the P-factor from S.pombe into CEN.PK2.
• Fluorescent cells in DAS
• Amplified all parts of the PUR system, but obtained vector-only clones after Gibson and transformation into E.coli