Difference between revisions of "Team:Chalmers-Gothenburg/Project Results"

Line 328: Line 328:
 
<p>• Fluorescent cells in DAS</p>
 
<p>• Fluorescent cells in DAS</p>
 
<p>• Amplified all parts of the PUR system, but obtained vector-only clones after Gibson and transformation into E.coli</p>
 
<p>• Amplified all parts of the PUR system, but obtained vector-only clones after Gibson and transformation into E.coli</p>
 +
<h2>DAS</h2>
 +
<p>The system without the amplification through dCAS9-VP64 (construct 4) was successfully assembled and integrated into S.cerevisiae CEN.PK2. The genomic integration was verified with colony PCR and sequencing. The detection system was initially intended to be integrated into IMFD-70, but as several transformations failed the strain was changed to CEN.PK2 instead.</p>

Revision as of 16:31, 18 September 2015



Add a banner to your wiki!

You can make the image 980px by 200px

Remember to call the file: "Team_Chalmers-Gothenburg_banner.jpg"

Overview

• Assembled and integrated two different safety switches, based on TPK2 overexpression or mRFP expression induced at low ATP levels, into CEN.PK2.

• SS TPK2 shows reduced growth rate after OD-measurement but no change in viability compared to wild type.

• mRFP measurements show that connection of pTEF1 to pSUC2 maintains the ATP repression mechanism of pSUC2, while achieving higher expression at low ATP levels.

• Successfully constructed and integrated the system to detect the P-factor from S.pombe into CEN.PK2.

• Fluorescent cells in DAS

• Amplified all parts of the PUR system, but obtained vector-only clones after Gibson and transformation into E.coli

DAS

The system without the amplification through dCAS9-VP64 (construct 4) was successfully assembled and integrated into S.cerevisiae CEN.PK2. The genomic integration was verified with colony PCR and sequencing. The detection system was initially intended to be integrated into IMFD-70, but as several transformations failed the strain was changed to CEN.PK2 instead.