Difference between revisions of "Team:Warwick/Description"
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<ul> <li> The J04450 gene from the registry was cloned into three different plasmids (pSB1K3, pSB3K3, and pSB4K4) each with a different copy number. </li> | <ul> <li> The J04450 gene from the registry was cloned into three different plasmids (pSB1K3, pSB3K3, and pSB4K4) each with a different copy number. </li> | ||
| − | <li>Each plasmid was transformed | + | <li>Each plasmid was transformed into electrocompetent MG1655-Z1</li> |
<li>Three colonies of each plasmid were then inoculated in LB and grown overnight.</li> | <li>Three colonies of each plasmid were then inoculated in LB and grown overnight.</li> | ||
<li>In the morning, each inoculation was refreshed in M9 + casein amino acid (500x dilution), induced with different concentrations of IPTG (0uM, 250uM, and 500uM IPTG) and grown for another 6 hours.</li> | <li>In the morning, each inoculation was refreshed in M9 + casein amino acid (500x dilution), induced with different concentrations of IPTG (0uM, 250uM, and 500uM IPTG) and grown for another 6 hours.</li> | ||
Revision as of 18:04, 18 September 2015