Difference between revisions of "Team:Pasteur Paris/Week 14"
Line 142: | Line 142: | ||
</table> | </table> | ||
</br> | </br> | ||
− | < | + | <ol style="text-align:left"> |
− | <li style="text-align:left">Midiprep of BBa_K808030</li> | + | <li style="text-align:left">Midiprep of BBa_K808030:</li> |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>Spe I and PstI enzymatic digest of BBa_K808007 with the phosphatase step. </li> | <li>Spe I and PstI enzymatic digest of BBa_K808007 with the phosphatase step. </li> | ||
Line 150: | Line 150: | ||
<li>DNA concentration assay using a spectrophotometer. </li> | <li>DNA concentration assay using a spectrophotometer. </li> | ||
</ul> | </ul> | ||
− | |||
</br> | </br> | ||
<table border="1" cellspacing="0" cellpadding="0" width="604"> | <table border="1" cellspacing="0" cellpadding="0" width="604"> | ||
Line 182: | Line 181: | ||
</table> | </table> | ||
</br> | </br> | ||
− | < | + | <ol style="text-align:left"> |
<li>Ligation of BBa_K808007 and BBa_K808030 </li> | <li>Ligation of BBa_K808007 and BBa_K808030 </li> | ||
<li>Transformation in electro-competent DH5-⍺ cells. </li> | <li>Transformation in electro-competent DH5-⍺ cells. </li> | ||
<li>Preparation for sequencing.</li> | <li>Preparation for sequencing.</li> | ||
− | |||
<br> | <br> | ||
<li style="text-align:left">Assembly of cluster 2:</li> | <li style="text-align:left">Assembly of cluster 2:</li> |
Revision as of 18:02, 18 September 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 14
^ |