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| − | <!-- Declare that you are going to use html code instead of wiki code --> | + | <h2>Introduction</h2> |
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| + | <p>The manufacturing process of biological products is complex and requires the use of living cells. Great progress has been made with industrial production techniques but contaminations are still a considerable problem the industry faces. Insufficient control of contaminations in bioreactors could compromise entire batches, resulting in high expenses. A contamination could lead to facilities or equipment having to be shut down for lengthy periods of time in order to conduct investigations and sterilize reactors. Ensuring that the bioreactors only contain the desired producing organism is critical to facility productivity, bioreactor throughput and product quality.</p> |
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| + | <p>We, <b>Team Chalmers Gothenburg</b>, have developed a novel strategy to detect and combat contaminations in continuous bioreactors, using <i>Saccharomyces cerevisiae</i> as the producing organism.</p> |
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| + | <p>The method for detection utilizes the pheromone pathway in <i>S. cerevisiae</i> where the GPC-receptor (Ste2) has been replaced with a fusion receptor, allowing the cells to detect ligands from contaminants. When a ligand binds to the fusion receptor it will activate a phosphorylation cascade within the cell, inducing an expression of <span style="color: #ff6699">red fluorescent proteins</span> that can be observed externally. The method for combating the detected contaminant is based on the use of UV-radiation, which effectively harms all living organisms. In order to prevent the producing cells from becoming inviable from the irradiation treatment, a DNA-repair system from the bacterium <i>Deinococcus radiodurans</i> is implemented into the cells. <i>D. radiodurans</i> is renowned for its extreme resistance to radiation, and our theory is that implementing these enzymes may increase <i>S. cerevisiae</i>'s resistance to radiation, allowing it to survive while the contaminant dies.</p> |
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| − | <div id ="mainContainer"><!--The closing tag for mainContainer should be placed at the bottom of each content page.-->
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| − | <div id="bannerContainer">
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| − | <br><br>
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| − | <h2> Add a banner to your wiki! </h2>
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| − | <p>You can make the image 980px by 200px</p>
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| − | <p> Remember to call the file: "<i>Team_Chalmers-Gothenburg_banner.jpg</i>" </p>
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| − | </div>
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| − | <!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg"><li>HOME</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Team"><li>TEAM
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Students"><li>Students</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Supervisors"><li>Supervisors</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Gallery"><li>Gallery</li></a>
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| − | </ul>
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| − | </li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project"><li>PROJECT
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Theory"><li>Theory</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Protocols"><li>Protocols</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Constructs"><li>Constructs</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/OtherApplications"><li>Other applications</li></a>
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| − | </ul>
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| − | </li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Achievements"><li>ACHIEVEMENTS
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project Results"><li>Project Results</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/BioBricks"><li>BioBricks</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Medals"><li>Medals</li></a>
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| − | </ul>
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| − | </li></a>
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| − | <a href="#"><li>MODELING
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Detection"><li>Detection</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety Switch"><li>Safety Switch</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Simulation"><li>Simulation</li></a>
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| − | </ul>
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| − | </li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Practices"><li>HUMAN PRACTICES
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/iGEMCommunity"><li>iGEM Community</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/NextGeneration"><li>Next Generation</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Society"><li>Society</li></a>
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| − | </ul></a></li>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Notebook"><li>NOTEBOOK
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Schedule"><li>Schedule</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>
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| − | </ul>
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| − | </li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety"><li>SAFETY
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/LabSafety"><li>Lab Safety</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Ethics"><li>Ethics</li></a>
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| − |
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| − | </ul>
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| − |
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| − | </li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Entrepreneurship"><li>THANKS
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| − | <ul>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Sponsors"><li>Sponsors</li></a>
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| − | <a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Acknowledgements"><li>Acknowledgements</li></a>
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| − | </ul>
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| − | </li></a>
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| − | </ul>
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| − | <h2> A study in <span style="color: #ff6699">Scarlet</span></h2>
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| − | <p id="pin">The manufacturing process of biological products is complex and requires the use of living cells. Great progress has been made with industrial production techniques but contaminations are still a considerable problem the industry faces. Insufficient control of contaminations in bioreactors could compromise entire batches, resulting in high expenses. A contamination could lead to facilities or equipment having to be shut down for lengthy periods of time in order to conduct investigations and sterilize reactors. Ensuring that the bioreactors only contain the desired producing organism is critical to facility productivity, bioreactor throughput and product quality.</p>
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| − | <p id="pin"> We, <b>Team Chalmers Gothenburg</b>, have developed a novel strategy to detect and combat contaminations in continuous bioreactors, using <i>Saccharomyces cerevisiae</i> as the producing organism.</p>
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| − |
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| − | <p id="pin">The method for detection utilizes the pheromone pathway in <i>S. cerevisiae</i> where the GPC-receptor (Ste2) has been replaced with a fusion receptor, allowing the cells to detect ligands from contaminants. When a ligand binds to the fusion receptor it will activate a phosphorylation cascade within the cell, inducing an expression of <span style="color: #ff6699">red fluorescent proteins</span> that can be observed externally. The method for combating the detected contaminant is based on the use of UV-radiation, which effectively harms all living organisms. In order to prevent the producing cells from becoming inviable from the irradiation treatment, a DNA-repair system from the bacterium <i>Deinococcus radiodurans</i> is implemented into the cells. <i>D. radiodurans</i> is renowned for its extreme resistance to radiation, and our theory is that implementing these enzymes may increase <i>S. cerevisiae</i>'s resistance to radiation, allowing it to survive while the contaminant dies.</p>
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| | <h2> A big thank you to our sponsors! </h2> | | <h2> A big thank you to our sponsors! </h2> |
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| | + | <img src="https://static.igem.org/mediawiki/2015/5/5d/AvancezChalmers_black_right.png" alt="Chalmers logo" width="300"> |
| | + | <img src="https://static.igem.org/mediawiki/2015/b/b6/Mastercard_logo.png" width="300"> |
| | + | <img src="https://static.igem.org/mediawiki/2015/f/f5/Promega-Logo_black.png"> |
| | + | <img src="https://static.igem.org/mediawiki/2015/b/b6/Geneious_logo.png"> |
| | + | <img src="https://static.igem.org/mediawiki/2015/a/a0/Qiagen_logo.png"> |
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| − | <a href="http://www.chalmers.se/en/Pages/default.aspx"><img src="https://static.igem.org/mediawiki/2015/5/5d/AvancezChalmers_black_right.png" alt="Chalmers logo" width="300" align="middle"></a>
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| − | <a href="http://www.chalmers.se/en/about-chalmers/alumni/chalmersmastercard/Pages/default.aspx"><img src="https://static.igem.org/mediawiki/2015/b/b6/Mastercard_logo.png" alt="Promega logo" width="40" align="middle"></a>
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| − | <a style="margin: 5px 50px;" href="http://www.promega.com"><img style='margin:20px;'src="https://static.igem.org/mediawiki/2015/f/f5/Promega-Logo_black.png" alt="Promega logo" width="40" align="middle"></a>
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| − | <a href="https://www.qiagen.com/"><img src="https://static.igem.org/mediawiki/2015/a/a0/Qiagen_logo.png" alt="Qiagen logo" width="40"></a>
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