Difference between revisions of "Team:Warwick/Results"

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<h5> sZF2 </h5>
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<ul> <li> A: Wild type DH5α-Z1 E. <i>coli</i> cells </li>
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<li> B: Uninduced Lpp_OmpA-sZF2 transformed cells</li>
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<li> C: Induced Lpp_OmpA-sZF2 transformed cells</li>
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<h5> sZF10 </h5>
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<ul> <li> A: Wild type DH5α-Z1 E. <i>coli</i> cells </li>
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<li> B: Uninduced Lpp_OmpA-sZF10 transformed cells</li>
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<li> C: Induced Lpp_OmpA-sZF10 transformed cells</li>
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From the images, no fluorescence was detected on any of the slides. This could be caused by the protein not being expressed on the surface of the cell. A random mutation in the colony that these cells were grown from could be the cause. While this plasmid was sequenced successfully, the colony that these cells originate from came from a replating of the original successful colony.
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<h5> sZF14 </h5>
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<ul> <li> A: Wild type DH5α-Z1 E. <i>coli</i> cells </li>
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<li> B: Uninduced Lpp_OmpA-sZF14 transformed cells</li>
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<li> C: Induced Lpp_OmpA-sZF14 transformed cells</li>
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</ul>
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These induced cells are the most clearly fluorescent from the set that was measured. Based on the previous results we decided to alter the protocol for preparing cells for microscopy, instead of fixing the cells on a glass slide, these cells were in liquid medium on the slide. The alternative protocol can be found here. By comparing images A and C it is clear that the fluorescence is caused by the protein being expressed on the cell surface. Image B shows that the Pl_lac promoter did not leak and cause uninduced cells to express the protein in great enough numbers to be visible.
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Revision as of 18:48, 18 September 2015

Warwick iGEM 2015

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