Difference between revisions of "Team:Czech Republic/Goals"

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Build a synthetic haploids which are able of using the haploid specific yeast pheromone pathway after their mating.
 
  
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Assemble as many sets of orthogonal pheromones and receptors as possible.
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Display selected proteins on the surface of the yeast.
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Module 1 builds synthetic haploid strains with refactored mating loci that are conjugated to make a functional IOD. These strains have the wild-type mating phenotype and differentially express a reprogrammed signalling pathway in their diploid state proving the feasibility of the clone-free assembly concept.  
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* Constructed a set of reporter promoters for yeast cells
 +
* Characterized reporter promoters in all mating types
 +
* Designed and materialized synthetic MATa and MATx strains
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* Built a synthetic diploid strain with a functional yeast pheromone pathway
 +
* Demonstrated the correct functionality of yeast pheromone pathway in synthetic diploids
  
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Module 2 builds a set of orthogonal pheromones and receptors. These pheromone-receptor pairs enable specific localized signalling proving the feasibility of multichannel signal transmission underlying logic operations necessary for reliable diagnosis.
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* Constructed a set of yeast plasmids with different mating pheromones and their receptors and contributed to the Registry with 6 BioBricks
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* Verified the correct coupling of the receptors to the yeast pheromone mating pathway
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* Verified the correct expression and secretion of the different pheromones
 +
* Showed the orthogonality of the used receptors and pheromones
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Module 3 builds a set of location tags that recognize common tumor surface markers and agglutinate cell populations. Location tags displayed in the correct conformation strengthen cell-cell interactions to enable localization of signal transmission.
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* Expressed streptavidin, EpCAM, Anti-EpCAM scFv, c-Myc scFv and anti-HuA scFv on the surface of yeasts
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* Demonstrated the ability of our receptors to bind chosen markers
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* Monitored the dynamic binding of our receptors and their corresponding markers
 
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Revision as of 20:04, 18 September 2015

Goals



Module 1 builds synthetic haploid strains with refactored mating loci that are conjugated to make a functional IOD. These strains have the wild-type mating phenotype and differentially express a reprogrammed signalling pathway in their diploid state proving the feasibility of the clone-free assembly concept.

  • Constructed a set of reporter promoters for yeast cells
  • Characterized reporter promoters in all mating types
  • Designed and materialized synthetic MATa and MATx strains
  • Built a synthetic diploid strain with a functional yeast pheromone pathway
  • Demonstrated the correct functionality of yeast pheromone pathway in synthetic diploids


Module 2 builds a set of orthogonal pheromones and receptors. These pheromone-receptor pairs enable specific localized signalling proving the feasibility of multichannel signal transmission underlying logic operations necessary for reliable diagnosis.

  • Constructed a set of yeast plasmids with different mating pheromones and their receptors and contributed to the Registry with 6 BioBricks
  • Verified the correct coupling of the receptors to the yeast pheromone mating pathway
  • Verified the correct expression and secretion of the different pheromones
  • Showed the orthogonality of the used receptors and pheromones

Module 3 builds a set of location tags that recognize common tumor surface markers and agglutinate cell populations. Location tags displayed in the correct conformation strengthen cell-cell interactions to enable localization of signal transmission.

  • Expressed streptavidin, EpCAM, Anti-EpCAM scFv, c-Myc scFv and anti-HuA scFv on the surface of yeasts
  • Demonstrated the ability of our receptors to bind chosen markers
  • Monitored the dynamic binding of our receptors and their corresponding markers