Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"

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<h3>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h3>
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<h2> Add a banner to your wiki! </h2>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg"><li>HOME</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Team"><li>TEAM
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Students"><li>Students</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Supervisors"><li>Supervisors</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Gallery"><li>Gallery</li></a>
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project"><li>PROJECT
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<ul> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Theory"><li>Theory</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Constructs"><li>Constructs</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Protocols"><li>Protocols</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/OtherApplications"><li>Other applications</li></a>
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Achievements"><li>ACHIEVEMENTS
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Project Results"><li>Project Results</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/BioBricks"><li>BioBricks</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Medals"><li>Medals</li></a> 
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<a href="#"><li>MODELING
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            <ul>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Detection"><li>Detection</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety Switch"><li>Safety Switch</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Simulation"><li>Simulation</li></a> 
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Practices"><li>HUMAN PRACTICES
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/iGEMCommunity"><li>iGEM Community</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/NextGeneration"><li>Next Generation</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Society"><li>Society</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Notebook"><li>NOTEBOOK
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Schedule"><li>Schedule</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Attributions"><li>Attributions</li></a>   
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</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Safety"><li>SAFETY
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/LabSafety"><li>Lab Safety</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Ethics"><li>Ethics</li></a> 
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Entrepreneurship"><li>THANKS
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Sponsors"><li>Sponsors</li></a>
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<a href="https://2015.igem.org/Team:Chalmers-Gothenburg/Acknowledgements"><li>Acknowledgements</li></a> 
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<h1>BioBricks</h1>
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<h2>1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2</h2>
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<p>Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from <i>STE2</i> (<i>Saccharomyces cerevisiae</i>) and Pheromone P-factor receptor <i>MAM2</i> (<i>Schizosaccharomyces pombe</i>) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from <i>S.pombe</i> through the Pheromone pathway in </i>S.cerevisiae</i>.</p>
 
<p>Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from <i>STE2</i> (<i>Saccharomyces cerevisiae</i>) and Pheromone P-factor receptor <i>MAM2</i> (<i>Schizosaccharomyces pombe</i>) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from <i>S.pombe</i> through the Pheromone pathway in </i>S.cerevisiae</i>.</p>
  
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<h2>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h2>
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<h3>2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2</h3>
 
<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
 
<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.</p>
  
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<h2>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h2>
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<h3>3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1</h3>
 
<p>Promoter to TPI1.</p>
 
<p>Promoter to TPI1.</p>
  
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<h2>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h2>
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<h3>4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA</h3>
 
<p>Recombinase A from <i>Deinococcus radiodurans</i>. Used in DNA-repair mechanisms.  
 
<p>Recombinase A from <i>Deinococcus radiodurans</i>. Used in DNA-repair mechanisms.  
 
Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
 
Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
  
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<h2>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h2>
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<h3>5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB</h3>
 
<p>Single strand binding protein from <i>Deinococcus radiodurans</i>. Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>
 
<p>Single strand binding protein from <i>Deinococcus radiodurans</i>. Codon optimized for <i>Saccharomyces cerevisiae</i> and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p>

Revision as of 23:34, 18 September 2015

BioBricks

1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2

Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (Schizosaccharomyces pombe) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from S.pombe through the Pheromone pathway in </i>S.cerevisiae</i>.

2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.

3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1

Promoter to TPI1.

4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA

Recombinase A from Deinococcus radiodurans. Used in DNA-repair mechanisms. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.

5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB

Single strand binding protein from Deinococcus radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.