Revision as of 20:10, 18 September 2015

Part Table

Source Organism	Gene	Part Name	Function	Characterised	Sequenced	Submitted
Expression vector “pAdapt” in pSB1C3	lacZalpha	BBa_K1807000	Used as cloning vector	✔	✔	✔
Escherichia coli	EcPPX	BBa_K1807001	Phosphate Kinase		✔	✔
Escherichia coli	EcPPK	BBa_K1807002	Phosphate Kinase		✔	✔
Escherichia coli	EcPstSCAB		Phosphate specific transporter		✔
Kingella oralis	KoPPK	BBa_K1807006	PolyPhosphate Kinase		✔	✔
Sinorhizobium meliloti	SmPstSCAB		Phosphate specific transporter	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPPK BA-91	BBa_K1807003	PolyPhosphate kinase	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPPK SK-12	BBa_K1807004	PolyPhosphate kinase	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPPK UW-1	BBa_K1807005	PolyPhosphate kinase	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPstSCAB	BBa_K1807007	Phosphate specific transporter		✔	✔

(Click to enlarge images)

This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the splice-in flanking sequences (they can be found in the design section).

This part codes for the exopolyphosphatase (PPX) enzyme of Escherichia coli. PPX is able to release phosphate residues from the ends of a polyphosphate chain.

This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain.

@@ Line 179: / Line 179: @@
 <div class="col-md-1"></div>
-<div class="col-md-12"></div>
+<div class="col-md-12">
 <h1>So what do all of these genes do?</h1>
@@ Line 277: / Line 277: @@
 </div>
+</div>
 </div>
 <div class="col-md-1"></div>