Difference between revisions of "Template:Heidelberg/project/standardization/validation"

 
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Figure 5. Switchable HRP DNAzyme for software validation
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HRP-mimicking DNAzyme activity is controlled by an aptamer that stems with part of the active site in absence of the ligand. In presence of the ligand the inhibiting stem is destabled and thus the HRP-mimicking DNAzyme's activity is recovered.
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Latest revision as of 02:42, 19 September 2015

Software Validation

Figure 5. Switchable HRP DNAzyme for software validation

HRP-mimicking DNAzyme activity is controlled by an aptamer that stems with part of the active site in absence of the ligand. In presence of the ligand the inhibiting stem is destabled and thus the HRP-mimicking DNAzyme's activity is recovered.

On top of that we needed a high-throughput validation system for the verification of our calculated aptamers. For this we also used the HRP-mimicking DNAzyme. The aptamers that were predicted by MAWS were fused with the HRP DNAzyme using JAWS to generate a bi-stable system (Fig.5). Thus the activity of the HRP DNAzyme became dependent on the presence of the ligand that can bind to the aptamer. This way we validated our aptamer candidates.