Difference between revisions of "Team:Aix-Marseille/Parts"
| Line 259: | Line 259: | ||
<br/><br/><br/><br/><br/> | <br/><br/><br/><br/><br/> | ||
<img src="https://static.igem.org/mediawiki/2015/6/60/Qsffff.png" class="img-responsive" width="475" height="325"> | <img src="https://static.igem.org/mediawiki/2015/6/60/Qsffff.png" class="img-responsive" width="475" height="325"> | ||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | <div class="space30"></div> | ||
| + | </div> | ||
| + | |||
| + | </section> | ||
| + | <section style="padding:30px 0px 50px;" class="arrow_box" id="team"> | ||
| + | |||
| + | <div class="container"> | ||
| + | <div class="row"> | ||
| + | <div class="col-md-6 left"> | ||
| + | <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div> | ||
| + | </h2></span></span> | ||
| + | <div class="space30"></div> | ||
| + | <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:<br/> | ||
| + | <ul> | ||
| + | <li>Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)</li> | ||
| + | <li>Cytochrome C P.Denitrificans - His Tag (BBa_S05317)</li> | ||
| + | <li>Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)</li> | ||
| + | <li>T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)</li> | ||
| + | </ul> | ||
| + | </div></p></span> | ||
| + | </div> | ||
| + | <div class="col-md-5 col-md-offset-1 col-sm-offset-1 space30"> | ||
| + | <br/><br/><br/><br/><br/> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/a/a7/Qsffqfqdf.png" class="img-responsive" width="475" height="325"> | ||
Revision as of 21:05, 18 September 2015
Parts
New biobricks using existing ones
We use existing biobrick to create new biobricks we needed:
- -T7 promoter with RBS (Bba_K525998) and optimized Laccase from T.Thermophilus (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)
- -T7 promoter with RBS and optimized Laccase from T.Thermophilus, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)
New biobricks we designed
We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:
- -Cytochrome C Synechocystis sp (BBa_K1601001), a cyanobacteria that suggests a cytochrome c
- -Cytochrome C of S.oneidensis (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.
- -Heme A from E.coli K12 (BBa_K1601002)that enables the heme overproduction
- -Yeast signal CXXCH from Synechocystis sp responsible for the S.oneidensis CytC translocation into the periplasm using TAT system (BBa_S05319)
- -Yeast signal CXXCH from Synechocystis sp responsible for the Synechocystis sp CytC translocation into the periplasm using TAT system (BBa_S05320)
Linkers we designed
We designed some linker to facilitate the meeting of the laccase and the cytochrom C
- Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC S.oneidensis (BBa_K1601008)
- Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC P.denitrificans (BBa_K1601011)
- Rigid and structured linker designed to link (or connect) T.Thermophilus and CytC Synechocystis sp(BBa_K1601013)
- Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC P.denitrificans (BBa_K1601009)
- Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC Synechocystis sp (BBa_K1601010)
- Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC S.oneidensis(BBa_K1601012)
New biobricks using existing ones
We use existing biobrick to create new biobricks we needed:
- -Yeast signal CXXCH from Synechocystis sp responsible for the S. oneidensis CytC translocation into the periplasm using TAT system
- -T7 promoter with RBS and Laccase E.coli (Bba_K863006) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601003)
- -T7 promoter with RBS and Laccase T.Thermophilus (Bba_K863011) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601004)
- -Cytochrome C Heme Lyase (CCHL)(BBa_K1601005)
- -Yeast signal CXXCH (BBa_K1601006)
- -Cytochrome C P.Denitrificans (BBa_K1601007)
- -Cytochrome C Synechocystis sp - His Tag (BBa_S05314)
- -Cytochrome C S.oneidensis - HisTag (BBa_S05315)
New biobricks using existing ones
We use existing biobrick to create new biobricks we needed:
- Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)
- Cytochrome C P.Denitrificans - His Tag (BBa_S05317)
- Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)
- T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)
