Revision as of 22:47, 18 September 2015

Parts

New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:

• -T7 promoter with RBS (Bba_K525998) and optimized Laccase from T.Thermophilus (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)


• -T7 promoter with RBS and optimized Laccase from T.Thermophilus, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)

New biobricks we designed

We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:

• -Cytochrome C Synechocystis sp (BBa_K1601001), a cyanobacteria that suggests a cytochrome c


• -Cytochrome C of S.oneidensis (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.


• -Heme A from E.coli K12 (BBa_K1601002)that enables the heme overproduction


• -Yeast signal CXXCH from Synechocystis sp responsible for the S.oneidensis CytC translocation into the periplasm using TAT system (BBa_S05319)


• -Yeast signal CXXCH from Synechocystis sp responsible for the Synechocystis sp CytC translocation into the periplasm using TAT system (BBa_S05320)

Linkers we designed

We designed some linker to facilitate the meeting of the laccase and the cytochrom C

• Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC S.oneidensis (BBa_K1601008)


• Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC P.denitrificans (BBa_K1601011)


• Rigid and structured linker designed to link (or connect) T.Thermophilus and CytC Synechocystis sp(BBa_K1601013)


• Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC P.denitrificans (BBa_K1601009)


• Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC Synechocystis sp (BBa_K1601010)


• Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC S.oneidensis(BBa_K1601012)

New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:

• -Yeast signal CXXCH from Synechocystis sp responsible for the S. oneidensis CytC translocation into the periplasm using TAT system
• -T7 promoter with RBS and Laccase E.coli (Bba_K863006) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601003)
• -T7 promoter with RBS and Laccase T.Thermophilus (Bba_K863011) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601004)
• -Cytochrome C Heme Lyase (CCHL)(BBa_K1601005)
• -Yeast signal CXXCH (BBa_K1601006)
• -Cytochrome C P.Denitrificans (BBa_K1601007)
• -Cytochrome C Synechocystis sp - His Tag (BBa_S05314)
• -Cytochrome C S.oneidensis - HisTag (BBa_S05315)

New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:

• Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)
• Cytochrome C P.Denitrificans - His Tag (BBa_S05317)
• Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)
• T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)