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Revision as of 13:41, 7 July 2015
Timeline
Week 26 - Preparing for take-off
- Inventory of the lab supplies
- Pouring LB Agar plates
- Amplification of the pET-Duet-1 vector
- Assessment of the safety requirements
- Preparing stocks for antibiotics, glycerol, LB & MilliQ
Week 27 - The Clone Wars
- Linearizing pET-Duet-1 for Gibson Assembly
- Our first successful Gibson Assembly!
- Amplification of the pET-Duet-1 vector
- Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning