We used E. coli strain DH5a for plasmid replication and BL21 for high level expression of our protein of interest. We would like to build a non invasive home diagnostic kit for Dengue Fever. Engineered bacteria will produce scFv antibody which has ability against glycoprotein of DEN virus envelope which contained in urine as sample. Our part contains osmY coding reading region, histidine, TEV cleave site, and scFv antibody. osmY coding region will produce peptide as signal which work due to osmotic shock. Histidine as protein tag for the nickel column purification. TEV cleave site will cut the scFV antibody from other protein. scFv which contained in our part consist of light chain, heavy chain, and Myc tag. We work at laboratory which has safety level 2 procedures. We wear rubber hand gloves, google glasses, and safety jacket. We sterilize all the waste based on the safety procedures that we used then wash our hands after work. We will put our antibody inside a small enclosed device that ordinary people can buy, like a test pack for pregnancy. It will has a very low risk. It will not harm human health, because it will detects the envelope glycoprotein of Dengue Virus that present in urine. If it reveals any threats in the future, we will withdraw all the products from market and examine what is wrong with our product or its system that we used then we fix it. From the device, it will give a result form the test. If it is positive, we also build a software to record personal data from patient which will be saved in the central database of the Ministry of Health.