Difference between revisions of "Team:Glasgow/Project/Overview/Protocols"
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| − | <h2 style="margin-top:13vh;"> | + | <h2 style="margin-top:13vh;">E. coli strains</h2> |
| − | <p class="mainText"> | + | <p class="mainText">TOP10: |
| + | </br>• F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- | ||
| + | </br>• Used for cloning of unmethylated DNA (such as from the distribution plate, or DNA synthesised by IDT) | ||
| + | </br> | ||
| + | </br> | ||
| + | DH5α: | ||
| + | </br>• F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– | ||
| + | </br>• Used for assembly of plasmids, and to methylate DNA from TOP10 in order to transform into DS941 | ||
| + | </br> | ||
| + | </br> | ||
| + | DS941: | ||
| + | </br>• an MG1655 derivative | ||
| + | </br>• AB1157, recF, lacZM15, lacIq | ||
| + | </br>• Used as chassis | ||
| + | </br> | ||
| + | </br> | ||
| + | DS941.Z1: | ||
| + | </br>• an MG1655 derivative | ||
| + | </br>• AB1157, recF, lacZM15, lacIq | ||
| + | </br>• extra copy of TetR and LacI | ||
| + | </br>• Used for measurements when extra Lac repressor was required | ||
| + | |||
| + | </p> | ||
<body> | <body> | ||
Revision as of 21:53, 18 September 2015
E. coli strains
TOP10: • F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- • Used for cloning of unmethylated DNA (such as from the distribution plate, or DNA synthesised by IDT) DH5α: • F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– • Used for assembly of plasmids, and to methylate DNA from TOP10 in order to transform into DS941 DS941: • an MG1655 derivative • AB1157, recF, lacZM15, lacIq • Used as chassis DS941.Z1: • an MG1655 derivative • AB1157, recF, lacZM15, lacIq • extra copy of TetR and LacI • Used for measurements when extra Lac repressor was required