Difference between revisions of "Template:Team:TU Eindhoven/Timeline HTML"

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<ul class="activiteitlijst">
 
<ul class="activiteitlijst">
 
<li><span class="activiteit">Linearizing pET-Duet-1 for Gibson Assembly </span></li>
 
<li><span class="activiteit">Linearizing pET-Duet-1 for Gibson Assembly </span></li>
−
<li><span class="activiteit">Our first successful Gibson Assembly!</span></li>
+
<li><span class="activiteit">Our first Gibson Assembly!</span></li>
 
<li><span class="activiteit">Amplification of the pET-Duet-1 vector</span></li>
 
<li><span class="activiteit">Amplification of the pET-Duet-1 vector</span></li>
 
<li><span class="activiteit">Nde1 &amp; Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning</span></li>
 
<li><span class="activiteit">Nde1 &amp; Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning</span></li>
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<h3> - Et tu, pET-Duet? </h3>
 
<h3> - Et tu, pET-Duet? </h3>
 
<ul class="activiteitlijst">
 
<ul class="activiteitlijst">
 +
<li><span class="activiteit">Linearizing pET-Duet-1 for Gibson Assembly and debugging </span></li>
 
<li><span class="activiteit">Amplification of the inserts for traditional cloning</span></li>
 
<li><span class="activiteit">Amplification of the inserts for traditional cloning</span></li>
 
<li><span class="activiteit">Xbal &amp; Pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning</span></li>
 
<li><span class="activiteit">Xbal &amp; Pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning</span></li>

Revision as of 10:46, 9 July 2015





Timeline



Week 26

- Preparing for take-off


  • Inventory of the lab supplies
  • Pouring LB Agar plates
  • Amplification of the pET-Duet-1 vector
  • Assessment of the safety requirements
  • Preparing stocks for antibiotics, glycerol, LB & MilliQ


Week 27

- The Clone Wars


  • Linearizing pET-Duet-1 for Gibson Assembly
  • Our first Gibson Assembly!
  • Amplification of the pET-Duet-1 vector
  • Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning


Week 28

- Et tu, pET-Duet?

  • Linearizing pET-Duet-1 for Gibson Assembly and debugging
  • Amplification of the inserts for traditional cloning
  • Xbal & Pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning
  • Xba1 & Pst1 digestion of the inserts (MCS-1) for traditional cloning
  • Nde1 & Kpn1 digestion of the inserts (MCS-2) for traditional cloning
  • Ligation