Difference between revisions of "Team:Uppsala/Part Collection"
| Line 31: | Line 31: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>J23101 + B0034 + RhlA + B0034 + RhlB</td> | <td>J23101 + B0034 + RhlA + B0034 + RhlB</td> | ||
| − | <td></td> | + | <td>Composite part consisting of the RBS, BBa_B0034, and the red fluorescent protein, dTomato (BBa_K1688019). dTomato has not been bricked previously.</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 38: | Line 38: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>B0034 + RhlA + B0034 + RhlB</td> | <td>B0034 + RhlA + B0034 + RhlB</td> | ||
| − | <td></td> | + | <td>BBa_J61051, nahR with nahR and sal promoters (Pr and Psal), codes for the inducible repressor NahR, which suppresses Psal in the absence of salicylate. This biobrick makes it possible to regulate the expression of the red fluorescent protein, dTomato, and the modified D439A/M510L CueO laccase (ModLac) with attached N-His6 tag and C-export tag (HlyA, BBa_K554002).</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 45: | Line 45: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>B0034 + RhlA</td> | <td>B0034 + RhlA</td> | ||
| − | <td></td> | + | <td>A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 52: | Line 52: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>B0034 + RhlB</td> | <td>B0034 + RhlB</td> | ||
| − | <td></td> | + | <td>Assembly of Anderson promoter BBa_J23110 and RBS + ModLac with HlyA tag (BBa_K1688006). ModLac is a modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.</td> |
<td>Biosurfers</td> | <td>Biosurfers</td> | ||
</tr> | </tr> | ||
| Line 73: | Line 73: | ||
<td>Coding</td> | <td>Coding</td> | ||
<td>ModLac with export tag</td> | <td>ModLac with export tag</td> | ||
| − | <td></td> | + | <td>Assembly of Anderson promoter BBa_J23101 and RBS + dTomato (BBa_K1688004). dTomato has not been bricked previously.</td> |
<td>Laccalicious</td> | <td>Laccalicious</td> | ||
</tr> | </tr> | ||
Revision as of 23:17, 18 September 2015
Part collection
| Biobrick Code | Type | Length | Description | Designers |
|---|---|---|---|---|
| BBa_K1688004 | Coding | J23101 + B0034 + RhlA + B0034 + RhlB | Composite part consisting of the RBS, BBa_B0034, and the red fluorescent protein, dTomato (BBa_K1688019). dTomato has not been bricked previously. | Biosurfers |
| BBa_K1688005 | Coding | B0034 + RhlA + B0034 + RhlB | BBa_J61051, nahR with nahR and sal promoters (Pr and Psal), codes for the inducible repressor NahR, which suppresses Psal in the absence of salicylate. This biobrick makes it possible to regulate the expression of the red fluorescent protein, dTomato, and the modified D439A/M510L CueO laccase (ModLac) with attached N-His6 tag and C-export tag (HlyA, BBa_K554002). | Biosurfers |
| BBa_K1688006 | Coding | B0034 + RhlA | A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus. | Biosurfers |
| BBa_K1688007 | Coding | B0034 + RhlB | Assembly of Anderson promoter BBa_J23110 and RBS + ModLac with HlyA tag (BBa_K1688006). ModLac is a modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus. | Biosurfers |
| BBa_K1688008 | Coding | B0034 + dTomato | An RBS assembled with the dTomato coding sequence, expressing a modified RFP. | Laccalicious |
| BBa_K1688009 | Coding | NahR+ dTomato + ModLac | The regulatory NahR construct assembled with the coding sequence for dTomato, as well as the coding sequence for the modified laccase. | Laccalicious |
| BBa_K1688010 | Coding | ModLac with export tag | Assembly of Anderson promoter BBa_J23101 and RBS + dTomato (BBa_K1688004). dTomato has not been bricked previously. | Laccalicious |
| BBa_K1688011 | Coding | J23101 + ModLac with export tag | A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag. | Laccalicious |
| BBa_K1688012 | Coding | J23101 + ModLac with export tag | A constitutive Anderson promoter assembled with the coding sequence for the modified laccase without an export tag. | Laccalicious |