Difference between revisions of "Team:Evry/Project/SurfaceDisplay"

Line 64: Line 64:
 
<h3>Surface display results</h3>
 
<h3>Surface display results</h3>
 
<p class="text-justify"> Before displaying our tumor antigen fused to the scFv DEC205, we first cloned the fusion protein AGA2P-GFP in order to observe surface display of GFP with our without the coexpression AGA1P. We obtained a GFP signal located around the membrane only in presence of AGA1P coexpression. </p>
 
<p class="text-justify"> Before displaying our tumor antigen fused to the scFv DEC205, we first cloned the fusion protein AGA2P-GFP in order to observe surface display of GFP with our without the coexpression AGA1P. We obtained a GFP signal located around the membrane only in presence of AGA1P coexpression. </p>
 
 
<div class="row">
 
<div class="row">
 
<div class="col-md-4">
 
<div class="col-md-4">
<p class="text-justify"><strong> Plasmids with the constructions :</strong></p>
+
<p class="text-justify"><strong>GFP fused to AGA2P observed in fluorescence microscopy. </strong></p>
<p class="text-justify"> A) AGA1P (B) AGA2P-OVA1-DEC205 (C) AGA2P-OVA1 (D) AGA2P-DEC205</p>
+
<p class="text-justify"> A) without AGA1P (B) with AGA1P (C) with AGA1P with Z-scale on one single yeast.</p>
 
</div>
 
</div>
 
<div class="col-md-8"><img border="0" class='img-responsive' src="https://2015.igem.org/File:GFPsurfacedisplay.jpg" alt="" /></div>
 
<div class="col-md-8"><img border="0" class='img-responsive' src="https://2015.igem.org/File:GFPsurfacedisplay.jpg" alt="" /></div>
 
</div>
 
</div>
 +
 +
<p class="text-justify"> Next, we cloned the full fusion protein OVA1-DEC205-HAtag inside our yeast with AGA1P. It was correctly displayed on yeast membrane (figure 4). HA-tag was fused at the end C-terminal of the protein to ensure complete translation upon detection. In both experiment (figure 2), the yeast was transformed to express AGA2P-OVA1-DEC205-HAtag and labelled with antibody anti-HA conjugated to the fluorochrome emitting at 650 nm.</p>
 +
  
  

Revision as of 22:42, 18 September 2015

Scroll to top To top