Difference between revisions of "Team:Tuebingen/Description"
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<rect x="1000" y="120" rx="20" ry="20" width="800" height="510" style="fill:#b3b3b3;stroke:black;stroke-width:5;opacity:0.78" /> | <rect x="1000" y="120" rx="20" ry="20" width="800" height="510" style="fill:#b3b3b3;stroke:black;stroke-width:5;opacity:0.78" /> | ||
<foreignObject x="1000" y="120" width="800" height="510"> | <foreignObject x="1000" y="120" width="800" height="510"> | ||
− | <p xmlns="http://www.w3.org/1999/xhtml" style="font-size: | + | <p xmlns="http://www.w3.org/1999/xhtml" style="font-size:54px;font-weight:bold;color:black;">The expression of the Dronpa-Cre-Dronpa construct is under control of a promotor, that is influenced by a Sensor. |
Any Biosensor that is able to positively regulate a promotor can be used in our setting.</p> | Any Biosensor that is able to positively regulate a promotor can be used in our setting.</p> | ||
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Revision as of 22:55, 18 September 2015
Project Description
A Biosensor Memory Module: Cre Sensor
To achieve the construction of a reversibly activatable Cre recombinase we want to apply the caging mechanism described by Zhou et al [1]. This caging is performed by fusing a copy of a variant of the fluorescent protein Dronpa to both the C- and N-terminus of the Cre recombinase. Since this Dronpa variant is able to form monomers or dimers depending on illumination with light of different wavelengths, we hope that the dimerized form inhibits the activity of the Cre recombinase.
Because our system only needs the expression of the caged Cre construct to be dependent on a sensor, it can be combined with almost all Biosensors that include a means of transcriptional control. This gives the system a wide variety of possible applications, especially in the context of the work of other iGEM teams.
[1] Zhou, XX; Chung, HK; Lam, AJ & Lin, MZ. (2012). Optical control of protein activity by fluorescent protein domains. Science, 338(6108), 810-814.