Difference between revisions of "Team:WLC-Milwaukee"

Revision as of 22:50, 20 July 2015

Welcome to iGEM 2015!

Your team has been approved and you are ready to start the iGEM season!

Before you start:

Please read the following pages:

Styling your wiki

For this year's project the WLC-Milwaukee team is looking into phage therapy. Specifically we are looking for phages specific to the outer-membrane efflux pore protein tolC. tolC analogs are found in most gram-negative bacteria, and they have been implicated as an essential protein in an efflux-based resistance mechanism to certain types of antibiotics. Our project involves expressing tolC homologs from infectious strains of bacteria in a tolC knockout strain of E coli. We are using these transgenic E coli to search for bacteriophages specific to our selected gastrointestinal tract pathogen's versions of tolC. Our hope is that the phages specific for a pathogen's tolC can be used in combination with an antibiotic generally resisted via an efflux mechanism to put the pathogenic bacteria in a lose-lose dilemma: bacteria which try to efflux the antibiotics will expose themselves to the pathogen-specific phage, while those which do not express TolC will be weakened to the antibiotic therapy.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Click here to edit this page!

See tips on how to edit your wiki on the Template Documentation page.

Templates

This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the Template Documentation page.

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

State your accomplishments! Tell people what you have achieved from the start.
Be clear about what you are doing and how you plan to do this.
You have a global audience! Consider the different backgrounds that your users come from.
Make sure information is easy to find; nothing should be more than 3 clicks away.
Avoid using very small fonts and low contrast colors; information should be easy to read.
Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2015 calendar
Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples:

Uploading pictures and files

You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

CLICK HERE TO UPLOAD FILES

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 <h4> Styling your wiki </h4>
-<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+<p>For this year's project the WLC-Milwaukee team is looking into phage therapy. Specifically we are looking for phages specific to the outer-membrane efflux pore protein tolC. tolC analogs are found in most gram-negative bacteria, and they have been implicated as an essential protein in an efflux-based resistance mechanism to certain types of antibiotics. Our project involves expressing tolC homologs from infectious strains of bacteria in a tolC knockout strain of E coli. We are using these transgenic E coli to search for bacteriophages specific to our selected gastrointestinal tract pathogen's versions of tolC. Our hope is that the phages specific for a pathogen's tolC can be used in combination with an antibiotic generally resisted via an efflux mechanism to put the pathogenic bacteria in a lose-lose dilemma: bacteria which try to efflux the antibiotics will expose themselves to the pathogen-specific phage, while those which do not express TolC will be weakened to the antibiotic therapy.  </p>
 <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 </div>