Difference between revisions of "Team:Evry/Project/SurfaceDisplay"

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<p class="text-justify"> Before displaying our tumor antigen fused to the scFv DEC205, we first cloned the fusion protein AGA2P-GFP in order to observe surface display of GFP with our without the coexpression AGA1P. We obtained a GFP signal located around the membrane only in presence of AGA1P coexpression. </p>
 
<p class="text-justify"> Before displaying our tumor antigen fused to the scFv DEC205, we first cloned the fusion protein AGA2P-GFP in order to observe surface display of GFP with our without the coexpression AGA1P. We obtained a GFP signal located around the membrane only in presence of AGA1P coexpression. </p>
  
<img border="0" class='img-responsive' src="https://static.igem.org/mediawiki/2015/0/00/GFPsurfacedisplay.jpg" alt="" />
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<img border="0" class='img-responsive' src="https://static.igem.org/mediawiki/2015/d/da/Image_manquante.png" alt="" />
 
<p class="text-justify"><strong>GFP fused to AGA2P observed in fluorescence microscopy. </strong> A) without AGA1P (B) with AGA1P (C) with AGA1P with Z-scale on one single yeast.</p>
 
<p class="text-justify"><strong>GFP fused to AGA2P observed in fluorescence microscopy. </strong> A) without AGA1P (B) with AGA1P (C) with AGA1P with Z-scale on one single yeast.</p>
  

Revision as of 23:24, 18 September 2015

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