Difference between revisions of "Team:William and Mary/Interlab Study"
Adhalleran (Talk | contribs) |
Adhalleran (Talk | contribs) |
||
| Line 152: | Line 152: | ||
<p class="description"></p> | <p class="description"></p> | ||
<p>We participated in the Interlab Measurement Study! We created are fluorescent constructs using DNA synthesis and Gibson Assembly. The constructs we measured were J23101 + I13504, J23106 + I13504, and J23117 + I13504. | <p>We participated in the Interlab Measurement Study! We created are fluorescent constructs using DNA synthesis and Gibson Assembly. The constructs we measured were J23101 + I13504, J23106 + I13504, and J23117 + I13504. | ||
| − | |||
We assembled the gBlocks using our <a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:Gibson">Gibson assembly protocol</a>, and imaged the constructs using our <a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:Imaging">imaging protocol</a>.</p> | We assembled the gBlocks using our <a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:Gibson">Gibson assembly protocol</a>, and imaged the constructs using our <a href = "https://2015.igem.org/Team:William_and_Mary/Protocols:Imaging">imaging protocol</a>.</p> | ||
<p> We created our fluorescent constructs using DNA synthesis and Gibson Assembly. After we transformed our assembled constructs into cells, we inoculated individual colonies in separate inoculation tubes and grew them in 5mL of LB containing chloramphenicol at 37*C overnight. Cells were imaged following our imaging protocol: link here. Cells were identified using Nikon Elements Software version 7.1 using binary thresholding on the GFP channel. Nikon Elements Software was then used to calculate mean fluorescence per cell and data was exported to Excel for final data analysis. </p> | <p> We created our fluorescent constructs using DNA synthesis and Gibson Assembly. After we transformed our assembled constructs into cells, we inoculated individual colonies in separate inoculation tubes and grew them in 5mL of LB containing chloramphenicol at 37*C overnight. Cells were imaged following our imaging protocol: link here. Cells were identified using Nikon Elements Software version 7.1 using binary thresholding on the GFP channel. Nikon Elements Software was then used to calculate mean fluorescence per cell and data was exported to Excel for final data analysis. </p> | ||
Revision as of 23:27, 18 September 2015
Interlab Study We participated in the Interlab Measurement Study! We created are fluorescent constructs using DNA synthesis and Gibson Assembly. The constructs we measured were J23101 + I13504, J23106 + I13504, and J23117 + I13504.
We assembled the gBlocks using our Gibson assembly protocol, and imaged the constructs using our imaging protocol. We created our fluorescent constructs using DNA synthesis and Gibson Assembly. After we transformed our assembled constructs into cells, we inoculated individual colonies in separate inoculation tubes and grew them in 5mL of LB containing chloramphenicol at 37*C overnight. Cells were imaged following our imaging protocol: link here. Cells were identified using Nikon Elements Software version 7.1 using binary thresholding on the GFP channel. Nikon Elements Software was then used to calculate mean fluorescence per cell and data was exported to Excel for final data analysis. Our data are shown below:
