Difference between revisions of "Team:Tec-Monterrey/Notebook"

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Revision as of 23:34, 18 September 2015


iGEM MTY 2015

Sun Mon Tue Wed Thu Fri Sat
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Miniprep (Dania, Hiyam) Digestion (Carolina) Electrophoresis (David, Alex)
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Stock RFP (Dania)
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25
Miniprep (Hiyam, Dania) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Ausencio, Dania, Hiyam)
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Miniprep (Ausencio, Dania, Hiyam) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Karen Carrizales, Dania)
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Calcium-competent cells (Jesús)
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Transformation (David, Ernesto)
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Inoculations (Alejandro, Carolina, Jesús, Dania)
30
Miniprep (David, Alejandro) Digestion (Alejandro, Hiyam) Electrophoresis (Alejandro, Ernesto) Digestion (Alejandro, David, Ernesto) Electrophoresis (David, Ernesto)
Sun Mon Tue Wed Thu Fri Sat
1
Purification (David, Minerva, Ernesto) Digestions (Carolina, Karen Carrizales) Electrophoresis (Carolina, Dania, Ausencio) Ligations (Jesús, Alex, Hiyam)
2
Purification (Carolina, Alejandro) Ligations (Carolina, Alejandro) Transformation (David, Karen Carrizales)
3
Stock P4 (Hiyam) Miniprep (Hiyam, Carolina)
4
Digestion (Hiyam, David) Electrophoresis (Carolina) Transformation (David, Jesús) Digestion (Karen Carrizales, Alejandro) Electrophoresis (Alejandro, Karen Carrizales, David) Purification (Hiyam, Dania) Ligations (Hiyam, Alejandro, Dania)
5
Transformation (Jesús, Carolina) Inoculations (David, Dania, Karen Carrizales, Alejandro)
6
Stocks P1, P2, P3, GFP (Carolina, Dania) Miniprep (Carolina, Dania, Ausencio) Inoculations (Jesus, Hiyam)
7
Degestions (Carolina, Dania, Hiyam) Miniprep (Hiyam, Jesús, David, Ausencio) Digestions (Ausencio, Dania, Hiyam)
8
Electrophoresis (David, Hiyam, Karen Carrizales, Dania) Purification (Karen Carrizales, Ausencio, David) Electrophoresis (Hiyam, Dania) Ligations (David, Karen Carrizales, Ernesto) Transformation (Minerva)
9
Electrophoresis (David, Ausencio, Ernesto) Digestion (David, Ernesto) Electrophoresis (David, Carolina, Dania) Purification (Hiyam, Dania) Ligations (David) Miniprep Lowe Budget (Alejandra, Karen Tello)
10
Digestion (Carolina) Electrophoresis (David, Ausencio) Transformation (David, Ausencio) Purification (Ausencio, David, Dania, Karen Carrizales) Digestion Low Budget (Alejandra, Karen Tello)
11
Ligations (Carolina)
12
Transformation (Carolina, Jesús, Ausencio)
13
Inoculations (Carolina, David, Karen Carrizales)
14
Miniprep (Carolina, Ausencio, David) Digestion (Hiyam, David) Transformation (Karen Carrizales, David) Electrophoresis (Hiyam, David)
15
Inoculations (Karen Carrizales, Jesús)
16
Miniprep (David, Hiyam) Digestion (David, Hiyam) Electrophoresis (Carolina, Karen Carrizales) Purification (Hiyam, Karen Carrizales, David)
17
Antibiotic preparation (Hiyam, Carolina) Transformation (Karen Carrizales, David)
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19
Miniprep (David) Digestion (Karen Carrizales, Ausencio) Electrophoresis, Purification (Hiyam, David) Inoculations (Ausencio)
20
Miniprep (David, Hiyam) Miniprep Low Budget, Digestion Lowe Budget (Alejandra, Karen Tello)
21
Digestion (Hiyam, Alejandra, Claudia) Electrophoresis (Karen Carrizales, Hiyam) Miniprep (David) Digestion (Hiyam, David) Electrophoresis (Hiyam)
22
Calcium-competent cells (Claudia)
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Miniprep (Hiyam, Dania, Carolina) Calcium-competent cells (Claudia) Transformation, Stocks P1 P2 P3 TOP10 DH5alpha (Alejandro) Digestion (Hiyam, Caro, Dania) Electrophoresis (David, Karen Carrizales)
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26
27
Miniprep (Dania, Hiyam, Caro) Digestion, Electrophoresis (David, Alejandro) Digestion (Carolina, Alejandro) Sf9 cells counting and passing (Minerva)
28
29
Digestion (Carolina, David, Hiyam) Electrophoresis (Hiyam, Alejandro) Transformation (Alejandro) Sf9 cells counting and passing (Dania, Claudia)
30
Digestion (Hiyam, Dania, David) Electrophoresis (David, Hiyam, Carolina) Purification (Alejandro, Hiyam) Digestion (Alejandro, Hiyam) Electrophoresis (Hiyam, Alejandro, David)
31
Electrophoresis (Alejandro, David) Purification (Carolina, David) Digestion (Carolina, Alejandro, Hiyam, Dania) Electrophoresis (David)
Sun Mon Tue Wed Thu Fri Sat
1
Purification (Hiyam, Dania) Ligation (Hiyam, Alex, Dania) Inoculations (Dania, Hiyam, Alex, David) Transformation (Carolina, Hiyam, Alejandro)
2
Digestion, Electrophoresis (Alejandro) Calcium-competent cells (Minerva, David) Transformation (Alejandro, Hiyam) Sf9 cells counting and passing (Dania, Claudia)
3
Stocks C2, C4, C5, P4, pFastBac (Alejandro, Hiyam) Miniprep (David, Hiyam) Digestion (Hiyam, David, Dania) Calcium-competent cells (Jesús, Karen Carrizales), Electrophoresis (Carolina, Dania)
4
Digestion, Electrophoresis (Alejandro, Karen Carrizales, David)
5
Cell Lysis (Alejandro) Transformation (Hiyam, Alejandro, Karen Tello)
6
Ligation, Digestion (Hiyam, Alejandro) Miniprep (Hiyam)
7
Miniprep 1 (David) Miniprep 2 (Alejandro, Karen Tello) Digestion (Karen Tello, Lourdes, Jesús) Electrophoresis (Karen Tello, Lourdes) Sf9 cells counting, passing and criopreservation (Dania, Claudia)
8
9
10
Electrophoresis (Alejandro) Inoculations (Jesús, David)
11
Miniprep 1 (Carolina, Karen Carrizales) Miniprep 2 (David) Miniprep 3 (Karen Tello, Alejandro, Hiyam) Digestion (Hiyam) Bacmid extraction (David) Sf9 cells counting and passing (Dania, Claudia)
12
Electrophoresis, Miniprep (Hiyam) Electrophoresis (Karen Tello, Ausencio) Inoculations (Carolina) Transfection (David, Dania, Minerva)
13
Miniprep (Carolina) Transformation (Alejandro)
14
SDS-Page (David) Western Blot (Minerva, David)
15
Luminiscence readings, Biuret Assay (Alejandro) Sf9 cells counting and passing (Dania, Claudia)
16
Sf9 cells counting and passing (Dania, Claudia) SDS-Page (David) Western Blot (David, Minerva) Luminiscence readings (Minerva, Alejandro) Virus amplification (Jesús, Karen Carrizales, Claudia)
17
18
Calcium-competents (Alejandro, Hiyam, Ausencio) Transformation, Digestion, Electrophoresis (Hiyam, Karen Tello, Ausencio) Purification, Ligation (Hiyam, Carolina) Inoculations, Miniprep (Carolina, Karen Tello)
19
20
Sf9 cells counting and passing (Dania, Claudia)
21
22
Sf9 cells counting and passing (Dania, Claudia)
23
Minprep (David) Digestion (Carolina) Bacmid extraction (Karen Carrizales, Carolina) Electrophoresis (Carolina) Inoculations (Karen Tello, Carolina) Sf9 cells counting and passing (Dania, Claudia)
24
Transfection (David, Dania, Minerva)
25
Miniprep, Digestion, Electrophoresis, Gibson Assembly (Hiyam, Karen Tello, Ausencio) Bradford (Alejandro) Sf9 cells counting and passing (Dania, Claudia)
26
27
Digestion (Alejandro)
28
Miniprep, Digestion, Electrophoresis (Hiyam, Alejandro, Ausencio) Purification (Eduardo, Hiyam, Alejandro, Ausencio)
29
Gibson Assembly (Eduardo, Alejandro, Hiyam, Ausencio) Sf9 cells counting and passing (Dania, Claudia)
30
Viral plaque assay (Jesús, Karen Carrizales, Claudia)
31
Sf9 cells counting and passing (Dania, Claudia)
Sun Mon Tue Wed Thu Fri Sat
1
Sf9 cells counting and passing (Dania, Claudia)
2
3
4
Sf9 cells counting and passing (Dania, Claudia)
5
Digestion (Hiyam) Sf9 cells counting and passing (Dania, Claudia)
6
Sf9 cells counting and passing (Dania, Claudia) Viral plaque assay (Jesús, Karen Carrizales, Claudia)
7
Gibson Assembly (Hiyam) Sf9 cells counting and passing (Dania, Claudia)
8
Sf9 cells counting and passing (Dania, Claudia)
9
Gibson Assembly (Eduardo, Hiyam, Alejandro, Ausencio) Sf9 cells counting and passing (Dania, Claudia)
10
Sf9 cells counting and passing (Dania, Claudia) Transfection (David, Dania, Minerva)
11
Sf9 cells counting and passing (Dania, Claudia)
12
Sf9 cells counting and passing (Dania, Claudia
13
Viral plaque staining, viral plaque assay (Karen Carrizales, Jesús)
14
15
Digestion (Alejandro, Ausencio) Electrophoresis (David, Eduardo, Ausencio) IMAC chromatography (Karen Carrizales, Jesús, Alejandro) SDS-Page (David) Western Blot (David, Minerva)
16
Miniprep (Alejandro, Hiyam) SDS-Page, Western Blot (David, Minerva)
17
Confirmation of parts for submitting (Karen Tello, Ausencio, Hiyam, Alejandro, Eduardo)
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