Difference between revisions of "Team:UI Indonesia/project"

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document.getElementById("judul").innerHTML = "Grand Design";
 
document.getElementById("judul").innerHTML = "Grand Design";
     document.getElementById("isi").innerHTML = "<img src='https://static.igem.org/mediawiki/2015/2/24/UI_INDONESIA_GRANDDESIGN.png' width='500px'>";
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     document.getElementById("isi").innerHTML = "<img src='https://static.igem.org/mediawiki/2015/2/24/UI_INDONESIA_GRANDDESIGN.png' width='500px'><br>We design a device for gram positive bacteria. After discussion, we chose to use B. subtilis 168and E. coli BL21as our host. B. subtilis 168 is a model for gram positive toggle switch. E. coli BL21 and B. subtilis 168 are chosen for chassis for expression of SboA.<br>For grand design we designeda toggle switch with the spermicidal protein; Subtilosin A (SboA) in the system. In the other side of the switch, we designed a suicide protein: NdoA.<br>The promoter in the system are Phyperspank(Phs) and PxylA. Phyperspank is a constitutive promoter  for gram positive when the repressor protein, LacI is not present. When present, LacI bound to Phs, inhibiting the expression of the promoter. However, with addition of lactose or IPTG, LacI would bind to IPTG and detach from the promoter, causing the promoter to express the protein downstream.";
 
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}
  

Revision as of 00:04, 19 September 2015

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