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function myFunction2() {

document.getElementById("judul").innerHTML = "Grand Design";

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document.getElementById("isi").innerHTML = "<img src='https://static.igem.org/mediawiki/2015/2/24/UI_INDONESIA_GRANDDESIGN.png' width='500px'> We design a device for gram positive bacteria. After discussion, we chose to use B. subtilis 168and E. coli BL21as our host. B. subtilis 168 is a model for gram positive toggle switch. E. coli BL21 and B. subtilis 168 are chosen for chassis for expression of SboA. For grand design we designeda toggle switch with the spermicidal protein; Subtilosin A (SboA) in the system. In the other side of the switch, we designed a suicide protein: NdoA. The promoter in the system are Phyperspank(Phs) and PxylA. Phyperspank is a constitutive promoter for gram positive when the repressor protein, LacI is not present. When present, LacI bound to Phs, inhibiting the expression of the promoter. However, with addition of lactose or IPTG, LacI would bind to IPTG and detach from the promoter, causing the promoter to express the protein downstream. PxylA is another constitutive promoter for gram positive. PxylA is repressed by xylR. Xylose would bind to xylR and cause PxylA to express the protein downstream. We express xylR under the effect of Phs and express LacI under the effect of PxylA. Thus, when one promoter is active, it repress the other promoter. When PxylA is active, it express SboA, the spermicidal protein. It also express LacI which inhibit the other promoter, Phs. When induced with IPTG, LacI binds to IPTG and inhibition on Phs decrease. Thus protein downstream of Phs active, which is XylR. XylR repress PxylA, thus the expression of SboA stop, and the production of NdoA started. This Grand Design was the ideal design. However, we are not testing this device as a whole due to safety reason. The safety reason issue is the pathogenicity of the bacteria if we test this device as a whole. SboA Expression For characterization of SboA in E. coli, we use pET9a with T7 promoter. The plasmid was transformed to E. coli BL21 CP. Culture was grown overnight. 1/10 of the culture was transferred to TB medium of 5 mL. It was shaken for 2 hours. 500μL of the culture was taken as a control before induction, the rest was induced with final concentration of 1mM IPTG. Culture of 2 hours and 4 hours after induction was taken and centrifuged. The pellet and the supernatant was taken. <img src='http://https://static.igem.org/mediawiki/2015/7/74/UI_Indonesia_Granddesign2.png' width='500px'> Toggle Switch <img src='https://static.igem.org/mediawiki/2015/b/b6/UI_Indonesia_ToggleSwitvch.png' width='500px'>";

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document.getElementById("isi").innerHTML = "<img src='https://static.igem.org/mediawiki/2015/2/24/UI_INDONESIA_GRANDDESIGN.png' width='500px'> We design a device for gram positive bacteria. After discussion, we chose to use B. subtilis 168and E. coli BL21as our host. B. subtilis 168 is a model for gram positive toggle switch. E. coli BL21 and B. subtilis 168 are chosen for chassis for expression of SboA. For grand design we designeda toggle switch with the spermicidal protein; Subtilosin A (SboA) in the system. In the other side of the switch, we designed a suicide protein: NdoA. The promoter in the system are Phyperspank(Phs) and PxylA. Phyperspank is a constitutive promoter for gram positive when the repressor protein, LacI is not present. When present, LacI bound to Phs, inhibiting the expression of the promoter. However, with addition of lactose or IPTG, LacI would bind to IPTG and detach from the promoter, causing the promoter to express the protein downstream. PxylA is another constitutive promoter for gram positive. PxylA is repressed by xylR. Xylose would bind to xylR and cause PxylA to express the protein downstream. We express xylR under the effect of Phs and express LacI under the effect of PxylA. Thus, when one promoter is active, it repress the other promoter. When PxylA is active, it express SboA, the spermicidal protein. It also express LacI which inhibit the other promoter, Phs. When induced with IPTG, LacI binds to IPTG and inhibition on Phs decrease. Thus protein downstream of Phs active, which is XylR. XylR repress PxylA, thus the expression of SboA stop, and the production of NdoA started. This Grand Design was the ideal design. However, we are not testing this device as a whole due to safety reason. The safety reason issue is the pathogenicity of the bacteria if we test this device as a whole. SboA Expression For characterization of SboA in E. coli, we use pET9a with T7 promoter. The plasmid was transformed to E. coli BL21 CP. Culture was grown overnight. 1/10 of the culture was transferred to TB medium of 5 mL. It was shaken for 2 hours. 500μL of the culture was taken as a control before induction, the rest was induced with final concentration of 1mM IPTG. Culture of 2 hours and 4 hours after induction was taken and centrifuged. The pellet and the supernatant was taken. <img src='http://https://static.igem.org/mediawiki/2015/7/74/UI_Indonesia_Granddesign2.png' width='500px'> Toggle Switch <img src='https://static.igem.org/mediawiki/2015/b/b6/UI_Indonesia_ToggleSwitvch.png' width='500px'> To characterize the toggle switch, we swtich the SboA with GFP and NdoA with RFP. Single colony was streaked over LB agar with antibiotic. After 18 hours, the culture was induced with 1M IPTG and observed under UV. Other characterization method involve culture of overnight colony to TB broth. After 2 hours shaking, the culture was induced with 1mM final concentration of IPTG. Another culture was induced with 1% xylose. The result was observed under UV.

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";

}

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 function myFunction2() {
 document.getElementById("judul").innerHTML = "Grand Design";
-     document.getElementById("isi").innerHTML = "<img src='https://static.igem.org/mediawiki/2015/2/24/UI_INDONESIA_GRANDDESIGN.png' width='500px'><br>We design a device for gram positive bacteria. After discussion, we chose to use B. subtilis 168and E. coli BL21as our host. B. subtilis 168 is a model for gram positive toggle switch. E. coli BL21 and B. subtilis 168 are chosen for chassis for expression of SboA.<br>For grand design we designeda toggle switch with the spermicidal protein; Subtilosin A (SboA) in the system. In the other side of the switch, we designed a suicide protein: NdoA.<br>The promoter in the system are Phyperspank(Phs) and PxylA. Phyperspank is a constitutive promoter  for gram positive when the repressor protein, LacI is not present. When present, LacI bound to Phs, inhibiting the expression of the promoter. However, with addition of lactose or IPTG, LacI would bind to IPTG and detach from the promoter, causing the promoter to express the protein downstream.<br>PxylA is another constitutive promoter for gram positive. PxylA is repressed by xylR. Xylose would bind to xylR and cause PxylA to express the protein downstream.<br>We express xylR under the effect of Phs and express LacI under the effect of PxylA. Thus, when one promoter is active, it repress the other promoter. When PxylA is active, it express SboA, the spermicidal protein. It also express LacI which inhibit the other promoter, Phs. When induced with IPTG, LacI binds to IPTG and inhibition on Phs decrease. Thus protein downstream of Phs active, which is XylR. XylR repress PxylA, thus the expression of SboA stop, and the production of NdoA started.<br>This Grand Design was the ideal design. However, we are not testing this device as a whole due to safety reason. The safety reason issue is the pathogenicity of the bacteria if we test this device as a whole.<br><br>SboA Expression<br><br>For characterization of SboA in E. coli, we use pET9a with T7 promoter. The plasmid was transformed to E. coli BL21 CP. Culture was grown overnight. 1/10 of the culture was transferred to TB medium of 5 mL. It was shaken for 2 hours. 500μL of the culture was taken as a control before induction, the rest was induced with final concentration of 1mM IPTG. Culture of 2 hours and 4 hours after induction was taken and centrifuged. The pellet and the supernatant was taken.<br><img src='http://https://static.igem.org/mediawiki/2015/7/74/UI_Indonesia_Granddesign2.png' width='500px'><br><br>Toggle Switch<br><br><img src='https://static.igem.org/mediawiki/2015/b/b6/UI_Indonesia_ToggleSwitvch.png' width='500px'>";
+     document.getElementById("isi").innerHTML = "<img src='https://static.igem.org/mediawiki/2015/2/24/UI_INDONESIA_GRANDDESIGN.png' width='500px'><br>We design a device for gram positive bacteria. After discussion, we chose to use B. subtilis 168and E. coli BL21as our host. B. subtilis 168 is a model for gram positive toggle switch. E. coli BL21 and B. subtilis 168 are chosen for chassis for expression of SboA.<br>For grand design we designeda toggle switch with the spermicidal protein; Subtilosin A (SboA) in the system. In the other side of the switch, we designed a suicide protein: NdoA.<br>The promoter in the system are Phyperspank(Phs) and PxylA. Phyperspank is a constitutive promoter  for gram positive when the repressor protein, LacI is not present. When present, LacI bound to Phs, inhibiting the expression of the promoter. However, with addition of lactose or IPTG, LacI would bind to IPTG and detach from the promoter, causing the promoter to express the protein downstream.<br>PxylA is another constitutive promoter for gram positive. PxylA is repressed by xylR. Xylose would bind to xylR and cause PxylA to express the protein downstream.<br>We express xylR under the effect of Phs and express LacI under the effect of PxylA. Thus, when one promoter is active, it repress the other promoter. When PxylA is active, it express SboA, the spermicidal protein. It also express LacI which inhibit the other promoter, Phs. When induced with IPTG, LacI binds to IPTG and inhibition on Phs decrease. Thus protein downstream of Phs active, which is XylR. XylR repress PxylA, thus the expression of SboA stop, and the production of NdoA started.<br>This Grand Design was the ideal design. However, we are not testing this device as a whole due to safety reason. The safety reason issue is the pathogenicity of the bacteria if we test this device as a whole.<br><br>SboA Expression<br><br>For characterization of SboA in E. coli, we use pET9a with T7 promoter. The plasmid was transformed to E. coli BL21 CP. Culture was grown overnight. 1/10 of the culture was transferred to TB medium of 5 mL. It was shaken for 2 hours. 500μL of the culture was taken as a control before induction, the rest was induced with final concentration of 1mM IPTG. Culture of 2 hours and 4 hours after induction was taken and centrifuged. The pellet and the supernatant was taken.<br><img src='http://https://static.igem.org/mediawiki/2015/7/74/UI_Indonesia_Granddesign2.png' width='500px'><br><br>Toggle Switch<br><br><img src='https://static.igem.org/mediawiki/2015/b/b6/UI_Indonesia_ToggleSwitvch.png' width='500px'><br>To characterize the toggle switch, we swtich the SboA with GFP and NdoA with RFP. Single colony was streaked over LB agar with antibiotic. After 18 hours, the culture was induced with 1M IPTG and observed under UV.<br>Other characterization method involve culture of overnight colony to TB broth. After 2 hours shaking, the culture was induced with 1mM final concentration of IPTG. Another culture was induced with 1% xylose. The result was observed under UV.
+";
 }