Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Colony PCR (t102)"
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<div class="header"> | <div class="header"> | ||
<div class="field fw1">#</div> | <div class="field fw1">#</div> | ||
− | <div class="field | + | <div class="field fw3">Temperature</div> |
− | <div class="field | + | <div class="field fw1">Time</div> |
<div class="field fw7">Step</div> | <div class="field fw7">Step</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
<div class="field fw1">1</div> | <div class="field fw1">1</div> | ||
− | <div class="field | + | <div class="field fw3">98 °C</div> |
− | <div class="field | + | <div class="field fw1">5:00</div> |
<div class="field fw7">Initial denaturation</div> | <div class="field fw7">Initial denaturation</div> | ||
</div> | </div> | ||
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<div class="record"> | <div class="record"> | ||
<div class="field fw1">2</div> | <div class="field fw1">2</div> | ||
− | <div class="field | + | <div class="field fw3">98 °C</div> |
− | <div class="field | + | <div class="field fw1">0:10</div> |
<div class="field fw7">Denaturation</div> | <div class="field fw7">Denaturation</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
<div class="field fw1"></div> | <div class="field fw1"></div> | ||
− | <div class="field | + | <div class="field fw3">75 °C</div> |
− | <div class="field | + | <div class="field fw1">0:30</div> |
<div class="field fw7">Annealing</div> | <div class="field fw7">Annealing</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
<div class="field fw1"></div> | <div class="field fw1"></div> | ||
− | <div class="field | + | <div class="field fw3">72 °C</div> |
− | <div class="field | + | <div class="field fw1">1:30</div> |
<div class="field fw7">Extension</div> | <div class="field fw7">Extension</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
− | |||
<div class="field fw1"></div> | <div class="field fw1"></div> | ||
<div class="field fw3"></div> | <div class="field fw3"></div> | ||
+ | <div class="field fw1"></div> | ||
<div class="field fw7">repeat 35x</div> | <div class="field fw7">repeat 35x</div> | ||
</div> | </div> | ||
<div class="record"> | <div class="record"> | ||
<div class="field fw1">3</div> | <div class="field fw1">3</div> | ||
− | <div class="field | + | <div class="field fw3">72 °C</div> |
− | <div class="field | + | <div class="field fw1">5:00</div> |
<div class="field fw7"></div> | <div class="field fw7"></div> | ||
</div> | </div> |
Revision as of 00:35, 19 September 2015
00:00, 18 August 2015 - 00:00, 18 August 2015
Plates were checked for colonies. Plate with salt promoter in pSB1C3 contained colonies. 4 colonies were checked with colony PCR, therefore VF and VF2 primers were used.
#
Temperature
Time
Step
1
98 °C
5:00
Initial denaturation
2
98 °C
0:10
Denaturation
75 °C
0:30
Annealing
72 °C
1:30
Extension
repeat 35x
3
72 °C
5:00
Each transformation had at least one correct insert according to the PCR. This colony was grown in LB with corresponding antibiotics overnight at 37 °C.