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Revision as of 00:56, 19 September 2015

NOISE - W&M iGEM

Interlab Measurement Study

We participated in the Interlab Measurement Study! You can learn more about our process and results on our Interlab Study page.

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PenPal Program

Teams Participating:

Based on project and collaboration ideas.
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Total Matches:

We matched teams with as many other teams possible.
9

Amount of Fun Had:

On a scale of 1-100.
100

Collaborating with other teams

Inter-team collaboration is a hallmark of iGEM. We collaborated with three different teams in the 2015 iGEM season. We assisted the University of Maryland team with the Interlab Measurement Study. We also helped out the Cambridge-JIC team by supplying them with fluorescent constructs to serve as positive controls for their Project: OpenScope 3D Printed Fluorescent Microscope Initiative. Finally, we participated in a collaborative effort with the University of Georgia iGEM team to determine fluorescence values for mCherry proteins controlled under an archaeal RBS library.

Collaboration with Maryland

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In order to assist the University of Maryland iGEM team with the iGEM Interlab Measurement Study, we assembled J23117 + I13504 for them and sent them the plasmid. We did this using DNA Synthesis and Gibson Assembly methods.

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Collaboration With University of Georgia

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The William and Mary iGEM team participated in a collaborative effort (with the University of Georgia iGEM team) to determine fluorescence values for mCherry controlled under an archaeal RBS library. This was the first organized attempt to determine the impact of specific archaeal RBSes in protein expression. Since there is little existing framework for archaeal work, the collaboration effort promoted the utilization of methanogenic archaea or methanogens as a potential substitute host for situations when E. coli is not the logical choice. The study could lay the framework for a new archaea-based InterLab Study modeled after the existent iGEM-sponsored E. Coli InterLab Study. The team imaged 29 samples for the UGA iGEM team and recorded fluorescence measurements for the samples. Data was reported to the UGA iGEM team..

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Collaboration with Cambridge

Cambridge-JIC iGEM 2015 is working on Project OpenScope, an initiative to create a 3d-printable programmable digital microscope. Upon request, we provided them with replicates of fluorescent constructs which we created for the Interlab Measurement Study (J23106 + I13504 and J23117 + I13504). They wanted green fluorescent samples with which to test the imaging capabilities of their microscope and the collaboration allowed us the opportunity to confirm that our some of our InterLab constructs were actually fluorescing as they should. Cambridge was successful in visualizing the fluorescent constructs and sent us images.