Difference between revisions of "Team:Northeastern Boston/Description"

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<p>We then shifted towards adaption of a plasmid from the Chlamy Collection: pOpt_mVenus. By surrounding the first promoter with the iGEM prefix and suffix, we created an iGEM compatible protein expression <a href="https://2015.igem.org/Team:Northeastern_Boston/Design" target="_blank">plasmid</a>. In this way, teams can remove the suffix and replace it with a codon-optimized coding sequence for heterologous proteins of interest, or remove the promoter region entirely, testing alternate promoters and coding sequences upstream of a hygromycin B selection cassette.</p>
 
<p>We then shifted towards adaption of a plasmid from the Chlamy Collection: pOpt_mVenus. By surrounding the first promoter with the iGEM prefix and suffix, we created an iGEM compatible protein expression <a href="https://2015.igem.org/Team:Northeastern_Boston/Design" target="_blank">plasmid</a>. In this way, teams can remove the suffix and replace it with a codon-optimized coding sequence for heterologous proteins of interest, or remove the promoter region entirely, testing alternate promoters and coding sequences upstream of a hygromycin B selection cassette.</p>
  
<p>Genetic engineering of microalgae is not new. <i>C. reinhardtii</i>, in particular, has been explored as a platform for heterologous proteins for years, but to a far lesser extent than mammalian cells or higher-order plants. Although they're more difficult to work with than bacteria, microalgae are poised to disrupt in areas like biofuel, agriculture, and pharmaceuticals. In this way, <i>C. reinhardtii</i> represents the organism of the future.</p>
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<p>Genetic engineering of microalgae is not new. <i>C. reinhardtii</i>, in particular, has been explored as a platform for heterologous proteins for years, but to a far lesser extent than mammalian cells or higher-order plants. Although they're difficult to engineer, microalgae are poised to disrupt in areas like biofuel, agriculture, and pharmaceuticals. With their primary reliance on CO<sub>2</sub> their capacity for producing complex proteins, microalgae like <i>C. reinhardtii</i> represent the chassis of the future.</p>
  
 
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Revision as of 01:51, 19 September 2015

Overview

The Need

Some Solutions

A Green Safety Net

Cost/Benefit

Approach