Difference between revisions of "Team:Kent/Notebook"

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<h1 align="center">Notebook</h1>
 
<h1 align="center">Notebook</h1>
  
 +
<head>
 +
<style>
 +
.firstHeading {
 +
display:none;
 +
}
 +
 +
body {
 +
padding: 0px;
 +
margin: 0px;
 +
 +
}
 +
 +
[class*='col-'] {
 +
float: left;
 +
padding-right: 20px;
 +
}
 +
 +
.grid-pad {
 +
padding: 20px 0px 20px 20px;
 +
}
 +
 +
.sub-grid-pad {
 +
padding: 20px 0px 20px 0px;
 +
}
 +
 +
.grid-pad > .row > [class*='col-']:last-of-type, .grid-pad > [class*='col-']:last-of-type {
 +
padding-right: 20px;
 +
}
 +
 +
.col-1-1 {
 +
width: 100%;
 +
}
 +
 +
.col-1-2, .col-2-4, .col-3-6, .col-5-10 {
 +
width: 50%;
 +
}
 +
 +
.col-1-3, .col-2-6, .col-3-9 {
 +
width: 33.33%;
 +
}
 +
 +
.col-2-3, .col-4-6, .col-6-9 {
 +
width: 66.66%;
 +
}
 +
 +
.col-1-4, .col-2-8 {
 +
width: 25%;
 +
}
 +
 +
.col-3-4 {
 +
width: 75%;
 +
}
 +
 +
.col-1-5, .col-2-10 {
 +
width: 20%;
 +
}
 +
 +
.col-2-5, .col-4-10 {
 +
width: 40%;
 +
}
 +
 +
.col-3-5, .col-6-10 {
 +
width: 60%;
 +
}
 +
 +
.col-4-5, .col-8-10 {
 +
width: 80%;
 +
}
 +
 +
.col-1-6 {
 +
width: 16.66%;
 +
}
 +
 +
.col-5-6 {
 +
width: 83.33%;
 +
}
 +
 +
.col-1-7 {
 +
width: 14.28%;
 +
}
 +
 +
.col-2-7 {
 +
width: 28.57%;
 +
}
 +
 +
.col-3-7 {
 +
width: 42.85%;
 +
}
 +
 +
.col-4-7 {
 +
width: 57.14%;
 +
}
 +
 +
.col-5-7 {
 +
width: 71.42%;
 +
}
 +
 +
.col-6-7 {
 +
width: 85.71%;
 +
}
 +
 +
.col-1-8 {
 +
width: 12.5%;
 +
}
 +
 +
.col-2-8 {
 +
width: 25%;
 +
}
 +
 +
.col-3-8 {
 +
width: 37.5%;
 +
}
 +
 +
.col-4-8 {
 +
width: 50%;
 +
}
 +
 +
.col-5-8 {
 +
width: 62.5%;
 +
}
 +
 +
.col-6-8 {
 +
width: 75%;
 +
}
 +
 +
.col-7-8 {
 +
width: 87.5%;
 +
}
 +
 +
.col-1-9 {
 +
width: 11.11%;
 +
}
 +
 +
.col-2-9 {
 +
width: 22.22%;
 +
}
 +
 +
.col-4-9 {
 +
width: 44.44%;
 +
}
 +
 +
.col-5-9 {
 +
width: 55.55%;
 +
}
 +
 +
.col-7-9 {
 +
width: 77.77%;
 +
}
 +
 +
.col-8-9 {
 +
width: 88.88%;
 +
}
 +
 +
.col-1-10 {
 +
width: 10%;
 +
}
 +
 +
.col-3-10 {
 +
width: 30%;
 +
}
 +
 +
.col-7-10 {
 +
width: 70%;
 +
}
 +
 +
.col-9-10 {
 +
width: 90%;
 +
}
 +
 +
.grid:after {
 +
content: "";
 +
display: table;
 +
clear: both;
 +
}
 +
 +
*, *:after, *:before {
 +
-webkit-box-sizing: border-box;
 +
-moz-box-sizing: border-box;
 +
box-sizing: border-box;
 +
}
 +
 +
[class*='col-']:last-of-type {
 +
padding-right: 0;
 +
}
 +
 +
.show {
 +
background-color:#FFFFFF;
 +
color: #081D38;
 +
box-shadow: 0px 0px 25px #888888;
 +
}
 +
 +
 +
.hide {
 +
background-color: auto;
 +
}
 +
 +
#pad {
 +
padding: 5px;
 +
}
 +
 +
body {
 +
background-color:#DFEDFF;
 +
}
 +
 +
#head {
 +
font-family: 'Open Sans', sans-serif;
 +
}
 +
 +
#mainhead {
 +
font-family:'Ubuntu', sans-serif;
 +
font-size: 50px;
 +
}
 +
 +
a{text-decoration:none;}
 +
 +
#navbar > div > a > p.show {
 +
box-shadow: 10px 10px 5px #888888;
 +
}
 +
 +
.currentpage {
 +
background-color:#FFFFFF;
 +
color: #081D38;
 +
box-shadow: 0px 0px 25px #888888;
 +
}
 +
 +
#navbar > div > a :hover{
 +
background-color: #C1CDCd;
 +
color: #081D38;
 +
}
 +
 +
#navbar > div > a:active > p.show {
 +
box-shadow: 0px 0px 25px #888888;
 +
}
 +
 +
.image-wrapper
 +
{
 +
position: relative;
 +
}
 +
 +
.scale-image
 +
{
 +
height: 100%;
 +
width: 100%;
 +
}
 +
/* Styling for the title (Month and Year) of the calendar */
 +
div.title
 +
            {
 +
    font: x-large Verdana, Arial, Helvetica, sans-serif;
 +
        text-align: center;
 +
    height: 40px;
 +
    background-color: white;
 +
    color: black;
 +
    }
 +
/* Styling for the footer */
 +
div.footer
 +
            {
 +
  font: small Verdana, Arial, Helvetica, sans-serif;
 +
                text-align: center;
 +
    }
 +
/* Styling for the overall table */
 +
table
 +
            {
 +
    font: 100% Verdana, Arial, Helvetica, sans-serif;
 +
    table-layout: fixed;
 +
    border-collapse: collapse;
 +
    width: 100%;
 +
    }
 +
/* Styling for the column headers (days of the week) */
 +
th
 +
            {
 +
    padding: 0 0.5em;
 +
    text-align: center;
 +
    background-color:gray;
 +
    color:white;
 +
    }
 +
/* Styling for the individual cells (days) */
 +
td 
 +
            {   
 +
    font-size: medium;
 +
    padding: 0.25em 0.25em; 
 +
    width: 14%;
 +
    height: 80px;
 +
    text-align: left;
 +
    vertical-align: top;
 +
    }
 +
/* Styling for the date numbers */
 +
.date 
 +
            {   
 +
    font-size: large;
 +
    padding: 0.25em 0.25em; 
 +
    text-align: left;
 +
    vertical-align: top;
 +
    }
 +
</style>
 +
        <script>
 +
            var monthSelector = 0;
 +
function count(direction)
 +
            {
 +
                if (direction == "up")
 +
                {
 +
                    monthSelector++;
 +
                }
 +
                else if (direction == "down")
 +
                {
 +
                    monthSelector--;
 +
                }
 +
               
 +
                if (monthSelector > 6)
 +
                {
 +
                    monthSelector = 0;
 +
                }
 +
                else if (monthSelector < 0)
 +
                {
 +
                    monthSelector = 6;
 +
                }
 +
                console.log(monthSelector);
 +
                refresh();
 +
            };
 +
           
 +
            window.onload = function() {refresh()};
 +
           
 +
            function refresh()
 +
            {
 +
                var calendar = new Array(7);
 +
                for (var y = 0; y < 7; y++)
 +
                {
 +
                    calendar[y] = document.getElementById("month" + y.toString());
 +
                    if (monthSelector == y)
 +
                    {
 +
                        calendar[y].style.display = "table-row";
 +
                    }
 +
                    else
 +
                    {
 +
                        calendar[y].style.display = "none";
 +
}
 +
                }
 +
            };
 +
    </script>
 +
</head>
 
<table>  
 
<table>  
 
<tr>  
 
<tr>  

Revision as of 14:05, 9 July 2015


iGEM Kent 2015

Notebook

June

M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30

July

M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 2930 31

August

M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 2627 28 29 30
31

September

M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30



















Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight
  • Meeting with advisors to discuss progress

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Day 6 Wet lab 29/06/15

    Transforming linear PSB1C3

    Day 7 Wet lab 30/06/15

  • Miniprep of PSB1A3 plasmid
  • Gel electrophoresis of a large quantity of PSB1C3
  • Meeting with advisors to discuss progress

    • Day 8 Wet lab 01/07/15

  • Gel electrophoresis of the purified DNA extracted
  • Transformation of pSB1A3 circular plasmid to VS45 cells (competent)
  • Digest of all pSB1C3 plasmids

    • Day 9 Wet lab 02/07/15

  • Competent cells transformation efficiency
  • Mini-prep of 1MS340 to get pSB1C3 plasmid
  • Gel extraction of big digest
  • Quantification of the digest. Added sample buffer to each digest

    • Day 10 Wet lab 03/07/15

  • Transformation efficiency

    • Day 11 Wet lab 06/07/15

  • TOP10 cells containing pSB1A3 with limonene synthase were cultured overnight on AMP plates
  • Colonies of VS45 containing pSB1A3 were patched onto chloramphenicol plates
  • Overnight digest of miniprepped pSBIC3 using ECORI and PSTl

    • Day 12 Wet lab 07/07/15

  • An agarose gel of the overnight digest was ran, but no bands were visible
  • Set up overnight cultures of pSBIC3 and pSBIA3 to be miniprepped the next day
  • Set up an overnight digest of pSBIC3

    • Day 13 Wet lab 08/07/15

  • Agarose gel of overnight digest was ran
  • miniprep of pSBIA3 and pSBIC3

    • Day 14 Wet lab 09/07/15

    Agarose gel of digested pSBIA3 and pSBIC3