Difference between revisions of "Team:York/Protocols"

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<center><h2> Protocols </h2></center>
 
<center><h2> Protocols </h2></center>
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    <li class="active"><a href="#one" role="tab" data-toggle="tab">LB Media</a></li>
 
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    <li><a href="#two" role="tab" data-toggle="tab">LB Media Agar Plates </a></li>
 
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    <li><a href="#three" role="tab" data-toggle="tab">Plasmid Purification</a></li>
 
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    <li><a href="#gel" role="tab" data-toggle="tab">Gel Electrophoresis</a></li>
 
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    <li><a href="#four" role="tab" data-toggle="tab">Gel Extraction</a></li>
 
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    <li><a href="#five" role="tab" data-toggle="tab">SOC Media</a></li>
 
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    <li><a href="#six" role="tab" data-toggle="tab">Competent Cells</a></li>
 
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    <li><a href="#seven" role="tab" data-toggle="tab">PCR</a></li>
 
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    <li><a href="#eight" role="tab" data-toggle="tab">Ligation</a></li>
 
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    <li><a href="#nine" role="tab" data-toggle="tab">Digestion</a></li>
 
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    <li><a href="#ten" role="tab" data-toggle="tab">Transformation</a></li>
 
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<h2>Lysogeny Broth</h2>
 
<h2>Lysogeny Broth</h2>
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<li>Store at room temperature or +4°C.</li>
 
<li>Store at room temperature or +4°C.</li>
 
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Revision as of 14:37, 9 July 2015

Protocols

Lysogeny Broth

Materials

  • 10g of tryptone
  • 5g of yeast extract
  • 10g of NaCl
  • 1L of Deionized Water
  • 1M NaCl
  • 1M KOH

Procedure

  1. Use a container with at least double the volume of the liquid that you are making.
  2. Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
  3. Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
  4. Autoclave.
  5. Store at room temperature or +4°C.