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Revision as of 17:11, 18 November 2015


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The following safety guidelines were followed:

  • We used Escherichia coli (MG1655), a wild type strain which cannot survive outside laboratory conditions, to test all our constructs.
  • Mycobacterium tuberculosis is a pathogenic strain. It falls under Risk Group 3 organisms and hence we will be using Mycobacterium smegmatis, a harmless non-pathogenic strain for our proof-of-concept experiments.
  • Our device has termination sequence which causes cell death after the cells have reached a certain density
  • We filled all the iGEM safety form.
  • All biological material were autoclaved before disposal.
  • Fume hoods were used for corrosive reagents.
  • Chemical and biological spills,if any, were contained and disposed depending on their nature.
  • Standard risk management procedure were followed based on the Institute’s policy.

More details can be found at the Department of Biotechnology website: http://dbtbiosafety.nic.in/