Difference between revisions of "Team:York/Protocols"

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Revision as of 15:40, 9 July 2015

Protocols

Lysogeny Broth

Materials

  • 10g of tryptone
  • 5g of yeast extract
  • 10g of NaCl
  • 1L of Deionized Water
  • 1M NaCl
  • 1M KOH

Procedure

  1. Use a container with at least double the volume of the liquid that you are making.
  2. Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
  3. Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
  4. Autoclave.
  5. Store at room temperature or +4°C.