Difference between revisions of "Team:York/Protocols"
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Revision as of 10:56, 13 July 2015
Protocols
Lysogeny Broth
Materials
- 10g of tryptone
- 5g of yeast extract
- 10g of NaCl
- 1L of Deionized Water
- 1M NaCl
- 1M KOH
Procedure
- Use a container with at least double the volume of the liquid that you are making.
- Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
- Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
- Autoclave.
- Store at room temperature or +4°C.