Latest revision as of 15:59, 4 October 2015

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Introduction

Antibody issue

PAST

Process

Results

Practices

What is Next

"I've never managed to keep a journal longer than two weeks." - J. K. Rowling

Complete Journal

All our successes and major events is highlighted in the following. We also listed the most important iGEM dates as well as protocols and primer designs. Feel free to click through the boxes, check out our primer designs or read through the protocols for the Lab. The protocols can be accessed by clicking on the protocol number.

Major events

Month	Date	Event
Febuary	23.02.15	First meeting
March	20.03.15- 22.03.15	Trip to Langeland
	24.03.15	Project idea chosen
	25.03.15	Visiting Knud Villy Christiansen on TEK
April	01.04.15	First day in Lab
	10.04.15	CFO Easter Party
	15.04.15	Lasagna night
	24.04.15- 26.04.15	Danish Meetup
July	24.07.15- 26.07.15	Nordic Jamboree
	28.07.15	Presentation for UNF-camp
August	03.08.15	Visiting animal testing facility
	05.08.15	Wiki and Wine
September	14.09.15	iGEM Alumne - Dinner with the old iGEM members
	16.09.15	Sleepover 1.0 in the "Lab"
	19.09.15	Wiki-freeze Brunch
	20.09.15	Movie Night: Back to the Future!

Lab successes

Month	Date	Succes
April	02.04.15	Created our first bio-bricks T18 and T25: MG1655:pSB1C3-T18, MG1655:pSB1K3-T18, MG1655:pSB1C3-T25 and MG1655:pSB1K3-T25
May	11.05.15	Test with GFP using FAX 11/05-2015: MG1655:pSB1C3-T18-Linker-GFP and MG1655:pSB1C3-T25-Linker-GFP
August	17.08.15	Knockout creation: MG1655:ΔCyA
	19.08.15	Final construct of T25: MG1655:pSB1K3-T25-Target
	27.08.15	Final construct of T18: MG1655:pSB1A3-T18-Linker-Scaffold-Library-Scaffold-3xFLAG
	28.08.15	Interlab study, completed with FAX on the constructs: TOP10:pSB1C3-J23101-I13504, TOP10:pSB1C3-J23106-I13504, TOP10:pSB1C3-J23117-I13504
September	04.09.15	Receiving a peptide aptamer library of our own design from IDT
	08.09.15	Positive control: MG1655: pSB1C3-T18-Linker-Leucin Zipper + pSB1K3-T25-Linker-Leuzine Zipper
	17.09.15	Characterization: Northern blot on MG1655:pSB1C3-PcstA -GFP
	18.09.15	First peptide aptamer screening??

iGEM deadlines

Month	Date	Deadline
March	31.03.15	Team registration closes
July	15.07.15	Project Description
	31.07.15	Giant Jamboree registration closes
August	07.08.15	Track Selection
	07.08.15	Project title and abstract
	28.08.15	Interlab Study data due
September	18.09.15	Parts Submission
	18.09.15	Wiki-freeze
	24.09.15- 28.09.15	Giant Jamboree

Protocols

Number	Name	Description
000	Template	Template for all the protocols made by SDU iGEM 2015.
001	Linker GFP	Assembly of a biobrick that contains the Linker-GFP gene.
002	Control of Leucine Zipper	To conjugate a leucin zipper to the T18 and T25 domain as used in the bacterial two-hybrid system and to furthermore verify that the bacterial two-hybrid system works
003	Interlab Study	Measurement of GFP under influence of different promoters.
004	cAMP Indicator	To create a brick that can indicate the activation of CyaA in the two hybrid system, by colour expression when increased presence of cAMP.
005	CyaA Knockout	To create a knockout of the CyaA gene in MG1655. This is done to get a bacterial strain which we can use to use and test the Two-Hybrid System.
006	Characterization of PcstA	To better characterize the cAMPinducable promotor PcstA. Three experiments are designed and executed in this protocol.
007	The Two Hybrid System	To construct two bricks that interact with each other allowing CyaA activation. The activation only occurs if the proteins bound to CyaA interact with each other. This interaction allows CyaA to produce cAMP from ATP.
008	Peptide Aptamer Screening	To screen for peptide aptamers using the twohybrid system.

Primers

Number	Name	Sequence
001	pKT25_BB_F	5’-ATATGAATTCGCGGCCGCTTCTAGAGCTGGCACGACAGGTTTCCCG-3’
002	pKT25_BB_R	5’-ATATCTGCAGCGGCCGCTACTACTAGGATCCAGCCCGCCGCGT-3’
003	pUT18_BB_R	5’-ATATCTGCAGCGGCCGCTACTAGTAGGATCCGCGTTCCACTGCGCCC-3’
004	Linker-GFP_F	5’-CGCTTCTAGCGGGATCCGAAAATTTGTATTTTCAATCTGGTATGCGTAAAGGAGAAGAACTTTTCACT GG-3’
005	Con-DsRed_F	5’-CGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCAAAGAGGAGAAATACTAGATGG ATAGCACTGAGAACGTCATCAAGC-3’
006	Con-DsRed_R	5’-ATATCTGCAGCGGCCGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTACTG GAACAGGTGGTGGCGG-3’
007	GFP-mCherry_R/ GFP-DsRed_R	5’-GAGCTAGCCGTAAACTAGTATATAAACGCAGAAAGGCCCACCC-3’
008	GFP-mCherry_F/ GFP-DsRed_F	5’-CTGCGTTTATATACTAGTTTACGGCTAGCTCAGTCCTAGGG-3’
009	Con-mCherry_R	5’-ATATCTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCC-3’
010	Con-mCherry_F	5’-CGCTTCTAGCGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCAAAGAGGAGAAATACTAGATGG TGAGCAAGG-3’
011	cyaA_F	5’-GAGGTCTGGCAGTGGATCCT-3’
012	cyaA_R	5’-GAGTGGATTTGTGATCGCAGCG-3’
013	CyaA_del_F	5’-GGAATTTACAGAGAATAAACGGTGCTACACTTGTATGTAGCGCATCTTTCTGTGTAGGCTGGAGCTGC TTC-3’
014	cyaA_del_R	5’-GTTTCCGCTAAGATTGCATGCCGGATAAGCCTCGCTTTCCGGCACGTTCACATATGAATATCCTCCTT AG-3’
015	FWLeuZ-F	5’-CCCCGGATCCAAACCGTTCCAGTGCCG-3’
016	RSLeuZ-R	5’-ATATCTGCAGCGGCCGCTACTAGTAGAGATCTACACTAGCACTATCAGAGTTATTAAGC-3’
017	iGEM VF2	5’-CCACCTGACGTCTAAGAAAC-3’
018	iGEM VR	5’-ATTACCGCCTTTGAGTGAGC-3’
019	Zip_F	5’-CGCTTCTAGAGGGATCCGAAAATTTGTATTTTCAATCTGGTATGAAACAGCTGGAAGACAAAGTTGA-3’
020	Zip_R	5’-ATATCTGCAGCGGCCGCTACTAGTAACGTTCACCAACCAGTTTTTTCAGA-3’
021	H1F_CyaA_del	5’-GCATCCAGACTGTCATAGTTATTGGAC-3’
022	H1R_CyaA_del	5’-GAAAGATGCGCTACATACAAGTGTAGC-3’
023	H2F_CyaA_del	5’-TGAACGTGCCGGAAAGCGAG-3’
024	H2R_CyaA_del	5’-AAGTGAGGAAATGCTGCGAGTATAAC-3’
025	GFP_LVA_F	5’-GCGCCGTCTAGAAAAGAGGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACT-3’
026	GFP_LVA_F	5’-GCCGGACTGCAGCGGCCGCTACTAGTATTAAGCTACTAAAGCGTAGTTTTCGTCG-3’

@@ Line 25: / Line 25: @@
 <table class="wikitable"  style="width:auto;">
   <tr>
-   <td><b>Month:</b></td>
+   <td><b>Month</b></td>
-   <td><b>Date:</b></td>
+   <td><b>Date</b></td>
-   <td><b>Event:</b></td>
+   <td><b>Event</b></td>
   </tr>
@@ Line 137: / Line 137: @@
 <tr>
-   <td><b>Month:</b></td>
+   <td><b>Month</b></td>
-   <td><b>Date:</b></td>
+   <td><b>Date</b></td>
-   <td><b>Succes:</b></td>
+   <td><b>Succes</b></td>
   </tr>
@@ Line 145: / Line 145: @@
    <td><b>April</b></td>
    <td>02.04.15</td>
-   <td><li>Created our first bio-bricks T18 and T25: MG1655:pSB1C3-T18, MG1655:pSB1K3-T18, MG1655:pSB1C3-T25 and MG1655:pSB1K3-T25</li></td>
+   <td>Created our first bio-bricks T18 and T25: MG1655:pSB1C3-T18, MG1655:pSB1K3-T18, MG1655:pSB1C3-T25 and MG1655:pSB1K3-T25</td>
   </tr>
@@ Line 151: / Line 151: @@
    <td><b>May</b></td>
    <td>11.05.15</td>
-   <td><li>Test with GFP using FAX 11/05-2015: MG1655:pSB1C3-T18-Linker-GFP and MG1655:pSB1C3-T25-Linker-GFP</li></td>
+   <td>Test with GFP using FAX 11/05-2015: MG1655:pSB1C3-T18-Linker-GFP and MG1655:pSB1C3-T25-Linker-GFP</td>
   </tr>
@@ Line 157: / Line 157: @@
    <td><b>August</b></td>
    <td>17.08.15</td>
-   <td><li>Knockout creation: MG1655:ΔCyA</li></td>
+   <td>Knockout creation: MG1655:ΔCyA</td>
   </tr>
@@ Line 163: / Line 163: @@
    <td><b></b></td>
    <td>19.08.15</td>
-   <td><li>Final construct of T25: MG1655:pSB1K3-T25-Target</li></td>
+   <td>Final construct of T25: MG1655:pSB1K3-T25-Target</td>
   </tr>
@@ Line 169: / Line 169: @@
    <td><b></b></td>
    <td>27.08.15</td>
-   <td><li>Final construct of T18: MG1655:pSB1A3-T18-Linker-Scaffold-Library-Scaffold-3xFLAG</li></td>
+   <td>Final construct of T18: MG1655:pSB1A3-T18-Linker-Scaffold-Library-Scaffold-3xFLAG</td>
   </tr>
@@ Line 175: / Line 175: @@
    <td><b></b></td>
    <td>28.08.15</td>
-   <td><li>Interlab study, completed with FAX on the constructs: TOP10:pSB1C3-J23101-I13504, TOP10:pSB1C3-J23106-I13504, TOP10:pSB1C3-J23117-I13504</li></td>
+   <td>Interlab study, completed with FAX on the constructs: TOP10:pSB1C3-J23101-I13504, TOP10:pSB1C3-J23106-I13504, TOP10:pSB1C3-J23117-I13504</td>
   </tr>
@@ Line 181: / Line 181: @@
    <td><b>September</b></td>
    <td>04.09.15</td>
-   <td><li>Receiving a peptide aptamer library of our own design from IDT</li></td>
+   <td>Receiving a peptide aptamer library of our own design from IDT</td>
   </tr>
@@ Line 187: / Line 187: @@
    <td><b></b></td>
    <td>08.09.15</td>
-   <td><li>Positive control: MG1655: pSB1C3-T18-Linker-Leucin Zipper + pSB1K3-T25-Linker-Leuzine Zipper</li></td>
+   <td>Positive control: MG1655: pSB1C3-T18-Linker-Leucin Zipper + pSB1K3-T25-Linker-Leuzine Zipper</td>
   </tr>
@@ Line 193: / Line 193: @@
    <td><b></b></td>
    <td>17.09.15</td>
-   <td><li>Caraktezaition: Northern blot on MG1655:pSB1C3-PcstA -GFP</li></td>
+   <td>Characterization: Northern blot on MG1655:pSB1C3-PcstA -GFP</td>
   </tr>
@@ Line 199: / Line 199: @@
    <td><b></b></td>
    <td>18.09.15</td>
-   <td><li>First peptide aptamer screening??</li></td>
+   <td>First peptide aptamer screening??</td>
   </tr>
@@ Line 208: / Line 208: @@
                              <div class="accordionTitel">iGEM deadlines</div>
-                              <div class="pane current" style="height:250px;" >
+                              <div class="pane current" style="height:auto;" >
-<table style="width:100%">
+<table class="wikitable" style="width:100%">
 <tr>
-   <td><h3>Month:</h3></td>
+   <td><b>Month</b></td>
-   <td><h3>Date:</h3></td>
+   <td><b>Date</b></td>
-   <td><h3>Deadline:</h3></td>
+   <td><b>Deadline</b></td>
   </tr>
@@ Line 276: / Line 276: @@
                              <div class="accordionTitel">Protocols</div>
-                              <div class="pane" style="height:410px;" >
+                              <div class="pane" style="height:auto;" >
-<table style="width:100%">
+<table class="wikitable" style="width:100%">
 <tr>
-   <td><h3>Number:</h3></td>
+   <td><b>Number</b></td>
-   <td><h3>Name:</h3></td>
+   <td><b>Name</b></td>
-   <td><h3>Description</h3></td>
+   <td><b>Description</b></td>
   </tr>
@@ Line 288: / Line 288: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/e/e8/SDU_2015_Protocol_000_-_Template.pdf" target="_blank">000</a></b></td>
    <td><b>Template</b></td>
-   <td><li>Template for all the protocols made by SDU iGEM 2015.</li></td>
+   <td>Template for all the protocols made by SDU iGEM 2015.</td>
   </tr>
@@ Line 294: / Line 294: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/3/39/SDU_2015_Protocol_001_-_Linker_GFP.pdf" target="_blank">001</a></b></td>
    <td><b>Linker GFP</b></td>
-   <td><li>Assembly of a biobrick that contains the Linker-GFP gene.</li></td>
+   <td>Assembly of a biobrick that contains the Linker-GFP gene.</td>
   </tr>
@@ Line 300: / Line 300: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/9/9a/SDU_2015_Protocol_002_-_Control_of_LeuZ.pdf" target="_blank">002</a></b></td>
    <td><b>Control of Leucine Zipper</b></td>
-   <td><li>To conjugate a leucin zipper to the T18 and T25 domain as used in the bacterial<br> two-hybrid
+   <td>To conjugate a leucin zipper to the T18 and T25 domain as used in the bacterial<br> two-hybrid
-system and to furthermore verify that the bacterial two-hybrid system<br> works</li></td>
+system and to furthermore verify that the bacterial two-hybrid system<br> works</td>
   </tr>
@@ Line 307: / Line 307: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/5/5a/SDU_2015_Protocol_003_-_Interlab_Study.pdf" target="_blank">003</a></b></td>
    <td><b>Interlab Study</b></td>
-   <td><li>Measurement of GFP under influence of different promoters.</li></td>
+   <td>Measurement of GFP under influence of different promoters.</td>
   </tr>
@@ Line 313: / Line 313: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/b/ba/SDU_2015_Protocol_004_-_cAMP_Indicator.pdf" target="_blank">004</a></b></td>
    <td><b>cAMP Indicator</b></td>
-   <td><li>To create a brick that can indicate the activation of CyaA in the two hybrid<br> system, by colour
+   <td>To create a brick that can indicate the activation of CyaA in the two hybrid<br> system, by colour
-expression when increased presence of cAMP.</li></td>
+expression when increased presence of cAMP.</td>
 </tr>
@@ Line 320: / Line 320: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/c/c5/SDU_2015_Protocol_005_-_CyaA_Knockout.pdf" target="_blank">005</a></b></td>
    <td><b>CyaA Knockout</b></td>
-   <td><li>To create a knockout of the CyaA gene in MG1655. This is done to get a bacterial<br> strain which we
+   <td>To create a knockout of the CyaA gene in MG1655. This is done to get a bacterial<br> strain which we
-can use to use and test the Two-Hybrid System.</li></td>
+can use to use and test the Two-Hybrid System.</td>
   </tr>
@@ Line 327: / Line 327: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/5/52/SDU_2015_Characterization_of_PcstA.pdf" target="_blank">006</a></b></td>
    <td><b>Characterization of PcstA</b></td>
-   <td><li>To better characterize the cAMPinducable promotor PcstA. Three experiments are designed and executed in this protocol.</li></td>
+   <td>To better characterize the cAMPinducable promotor PcstA. Three experiments are designed and executed in this protocol.</td>
   </tr>
@@ Line 333: / Line 333: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/a/ac/SDU_2015_Protocol_007_-_Two-hybrid_system.pdf" target="_blank">007</a></b></td>
    <td><b>The Two Hybrid System</b></td>
-   <td><li>To construct two bricks that interact with each other allowing CyaA activation. The activation
+   <td>To construct two bricks that interact with each other allowing CyaA activation. The activation
 only occurs if the proteins bound to CyaA interact with each other. This interaction allows CyaA
-to produce cAMP from ATP.</li></td>
+to produce cAMP from ATP.</td>
   </tr>
@@ Line 341: / Line 341: @@
    <td><b><a class="pdfFile" href="https://static.igem.org/mediawiki/2015/5/59/SDU_2015_Protocol_008_-_Peptide_Aptamer_Screening.pdf" target="_blank">008</a></b></td>
    <td><b>Peptide Aptamer Screening</b></td>
-   <td><li>To screen for peptide aptamers using the twohybrid system</li></td>
+   <td>To screen for peptide aptamers using the twohybrid system.</td>
   </tr>
@@ Line 350: / Line 350: @@
                               <div class="accordionTitel">Primers</div>
-                               <div class="pane" style="height:750px;" >
+                               <div class="pane" style="height:auto;" >
-<table style="width:100%">
+<table class="wikitable" style="width:100%">
   <tr>
-   <td><h3>Number:</h3></td>
+   <td><b>Number</b></td>
-   <td><h3>Name:</h3></td>
+   <td><b>Name</b></td>
-   <td><h3>Sequence:</h3></td>
+   <td><b>Sequence</b></td>
   </tr>

Difference between revisions of "Team:SDU-Denmark/Tour41"

Latest revision as of 15:59, 4 October 2015

Complete Journal