Difference between revisions of "Team:Vanderbilt/Parts/Repair Enzymes"

To build our genetically-engineered strain of bacteria with decreased susceptibility to mutation, we produced several parts for expression DNA repair enzymes. Each of these genes were themselves optimized by our software to have a minimal number of overall mutation hotspots. Included are enzymes for repairing UV-induced lesions, oxidative damage, and the enzymes necessary to construct our "incorruptible cell". Part numbers and their corresponding genes for each are listed below:

K1673201 T4 Pyrimidine Dimer Glycosylase (Mutation Optimized)

K1673202 Formamidopyrimidine DNA glycosylase (Mutation Optimized)

K1673203 S1 Nuclease (Overall Mutation Optimized)

K1673204 T7 Endonuclase (Mutation Optimized)

K1673205 T7 Endonuclease (Homology Optimized )

K1673213 IPTG Promoter Mutation Optimized S1 Nuclease

K1673214 IPTG Promoter Mutation Optimized T7 Endonuclease

K1673223 T7 Promoter Mutation Optimized S1 Nuclease

K1673224 T7 Promoter Mutation Optimized T7 Nuclease