Difference between revisions of "Team:TCU Taiwan/Modeling"
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| − | <span style="font-family:Calibri;text-align:justify;"><font size="4"></br> | + | <span style="font-family:Calibri;text-align:justify;align:left;"><font size="4"></br>Fig. 1 The pictures of cell migration in the Signiferin group at 0 hour and 24 hours</font></span> |
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| − | <span style="font-family:Calibri;text-align:justify;"><font size="4"></br> | + | <span style="font-family:Calibri;text-align:justify;align:left;"><font size="4"></br>Fig. 1-1 The percentage of migration distance between Signiferin group and mock</font></span> |
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| − | + | Conclusion: As Fig. 1 the Signiferin group with one ten dilution of crude extract, no significant differences of cell migration were observed. And we also compared with mock group (show as Fig. 1-1) there were no signify difference too. It means Signiferin doesn’t has ability to help wound healing. </font></span></h1> | |
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| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/b/bf/Tcu_Taiwan_REsult_0919_3_1.jpeg" width="450px" height="300px" align="center"> |
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| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/e/e5/Tcu_Taiwan_REsult_0919_3_2.jpeg" width="450px" height="300px" align="center"> |
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| − | In the | + | Conclusion: In the Epinecidin-1 group significant efficiency of migration were observed in the picture in Fig. 2. And we also compared the migration distance between Epinecidin-1 group and mock group. As Fig. 2-1 shows, wound healing time was cut in half, in other words, if a wound needs 4 weeks to recovered, now only needs 2 weeks. These results consistent with previous publications. </font></span></h1> |
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| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/4/46/Tcu_Taiwan_REsult_0919_2_1.jpeg" width="450px" height="300px" align="center"> |
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| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/b/b7/Tcu_Taiwan_REsult_0919_2_2.jpeg" width="450px" height="300px" align="center"> |
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Revision as of 08:14, 26 October 2015
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We had collected the data of disinfection experiments and in vitro wound healing assay. Than we analyzed it into charts to represent our experimental achievement. |
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We had tested the disinfection ability of our AMPs and proved our system is really worked.First, we prepared 1 ml LB medium of S. aureus concentration of OD 0.01 at 600 nm. Than we added 1 ml sample into tube and measured the OD at 600 nm once in two hours.The experiments were carried out for 24 hours, and the result were collected at 0, 2nd, 4th, 6th and 24th hours. We had analyzed the data and made it into chart. |
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Conclusions: As the chart we can see that both Signiferin and Epinecidin-1 have ability to kill S. aureus. At 0 hour, there were no obvious differences between control and experimental group. However, at 6th hours, inhibition of bacterial growth were obvious in both signiferin and epi-1. But the effect is not significant. We speculated the concentration is not enough to see the best efficiency. |
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After cell migration experiments, we use ImageJ to measure the distance of the migration then compared them to interpret our results. We had collected data and analyze it to made charts. |
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| Fig. 1 The pictures of cell migration in the Signiferin group at 0 hour and 24 hours | Fig. 1-1 The percentage of migration distance between Signiferin group and mock |
Conclusion: As Fig. 1 the Signiferin group with one ten dilution of crude extract, no significant differences of cell migration were observed. And we also compared with mock group (show as Fig. 1-1) there were no signify difference too. It means Signiferin doesn’t has ability to help wound healing. |
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| fig.2 HaCat cell tests for Epi-1 and Signiferin | fig.2-1 HaCat cell tests for Epi-1 and Signiferin |
Conclusion: In the Epinecidin-1 group significant efficiency of migration were observed in the picture in Fig. 2. And we also compared the migration distance between Epinecidin-1 group and mock group. As Fig. 2-1 shows, wound healing time was cut in half, in other words, if a wound needs 4 weeks to recovered, now only needs 2 weeks. These results consistent with previous publications. |
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| fig.3 HaCat cell tests for Epi-1 | fig.3-1 HaCat cell tests for Epi-1 |
In the this group, there are no significant result in the Mock and three control group. Additionally, the HaCat in 50l of Epi-1 rarefied LB medium, the Fig. 3-1, shows significant effectiveness. In other words, 50l Epi-1 has proven can help wound healing indirectly. |
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| Contact us tcutaiwan@gmail.com No.701, Sec. 3, Zhongyang Rd. Hualien 97004, Taiwan |